VPS27/YNR006W Summary Help

Standard Name VPS27 1, 2
Systematic Name YNR006W
Alias GRD11 3 , SSV17 , VPL23 4 , VPT27 1 , DID7 5
Feature Type ORF, Verified
Description Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) (6, 7)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXIV:636986 to 638854 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VPS27 GO evidence and references
  View Computational GO annotations for VPS27
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
571 total interaction(s) for 386 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 15
  • Biochemical Activity: 14
  • Co-crystal Structure: 2
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 3
  • Protein-peptide: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Negative Genetic: 407
  • Phenotypic Enhancement: 36
  • Phenotypic Suppression: 8
  • Positive Genetic: 30
  • Synthetic Growth Defect: 19
  • Synthetic Lethality: 2
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 622
Molecular Weight (Da) 70,973
Isoelectric Point (pI) 5.05
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:636986 to 638854 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1869 636986..638854 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005289
References cited on this page View Complete Literature Guide for VPS27
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Nothwehr SF, et al.  (1996) The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol 16(6):2700-7
4) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
5) Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
6) Piper RC, et al.  (1995) VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae. J Cell Biol 131(3):603-17
7) Bilodeau PS, et al.  (2002) The Vps27p Hse1p complex binds ubiquitin and mediates endosomal protein sorting. Nat Cell Biol 4(7):534-9