VPS27/YNR006W Summary Help

VPS27 BASIC INFORMATION

Standard Name VPS27 1, 2
Systematic Name YNR006W
Alias GRD11 3 , SSV17 , VPL23 4 , VPT27 1 , DID7 5
Feature Type ORF, Verified
Description Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) (6, 7)
Name Description Vacuolar Protein Sorting 1, 2
GO Annotations All VPS27 GO evidence and references
    View Computational GO annotations for VPS27
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All VPS27 Phenotype details and references
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Interactions VPS27 All interactions details and references
70 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • Biochemical Activity: 13
  • Co-crystal Structure: 2
  • Co-localization: 1
  • PCA: 1
  • Protein-peptide: 2
  • Two-hybrid: 9
Genetic Interactions
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 2
  • Synthetic Rescue: 4
Sequence Information
ChrXIV:636989 to 638857 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2010-01-05 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1869 636989..638857 2010-01-05 1997-01-28
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005289

VPS27 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for VPS27

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for VPS27]

1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Nothwehr SF, et al.  (1996) The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol 16(6):2700-7
4) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
5) Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
6) Piper RC, et al.  (1995) VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae. J Cell Biol 131(3):603-17
7) Bilodeau PS, et al.  (2002) The Vps27p Hse1p complex binds ubiquitin and mediates endosomal protein sorting. Nat Cell Biol 4(7):534-9