Standard Name	CIT1 1
Systematic Name	YNR001C
Alias	LYS6
Feature Type	ORF, Verified
Description	Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication (1, 2, 3) Also known as: CS1
Name Description	CITrate synthase 1

Chromosomal Location	ChrXIV:631061 to 629622 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for CIT1
Molecular Function
Manually curated	citrate (Si)-synthase activity (IDA, IMP)
Biological Process
Manually curated	acetyl-CoA catabolic process (IDA, IMP) citrate metabolic process (TAS) glutamate biosynthetic process (TAS) tricarboxylic acid cycle (TAS)
Cellular Component
Manually curated	mitochondrion (IDA, IPI)
High-throughput	mitochondrion (IDA)

Pathways	superpathway of TCA cycle and glyoxylate cycle TCA cycle, aerobic respiration

Classical genetics
null	zinc(2+) accumulation: decreased resistance to sirolimus: increased respiratory growth: decreased survival rate in stationary phase: decreased utilization of carbon source: absent
unspecified	utilization of carbon source: absent
Large-scale survey
null	competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased lifespan: increased mitochondrial genome maintenance: abnormal resistance to streptomycin: decreased resistance to cycloheximide: decreased resistance to CTBT: decreased respiratory growth: decreased rate respiratory growth: absent sporulation efficiency: decreased starvation resistance: decreased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: increased vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	93 total interaction(s) for 49 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 3 Co-localization: 2 Reconstituted Complex: 1
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 26 Phenotypic Enhancement: 8 Phenotypic Suppression: 6 Positive Genetic: 22 Synthetic Growth Defect: 7 Synthetic Lethality: 5 Synthetic Rescue: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIV:631061 to 629622 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1440 631061..629622 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005284