RPD3/YNL330C Summary Help

RPD3 BASIC INFORMATION

Standard Name RPD3 1
Systematic Name YNL330C
Alias REC3 2 , SDI2 3 , SDS6 4 , 5 , MOF6 6
Feature Type ORF, Verified
Description Histone deacetylase; regulates transcription and silencing; plays a role in regulating Ty1 transposition (7, 8 and see Summary Paragraph)
Name Description Reduced Potassium Dependency 1
GO Annotations All RPD3 GO evidence and references
    View Computational GO annotations for RPD3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All RPD3 Phenotype details and references
Classical genetics
null
unspecified
Large-scale survey
null
Interactions RPD3 All interactions details and references
503 total interaction(s) for 303 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 130
  • Affinity Capture-Western: 27
  • Co-purification: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 2
Genetic Interactions
  • Dosage Lethality: 13
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 91
  • Phenotypic Suppression: 44
  • Synthetic Growth Defect: 83
  • Synthetic Lethality: 69
  • Synthetic Rescue: 39
Sequence Information
ChrXIV:19302 to 18001 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -237 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1302 19302..18001 1996-07-31 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005274

RPD3 RESOURCES

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Expression Summary histogram

ADDITIONAL INFORMATION for RPD3

SUMMARY PARAGRAPH for RPD3

RPD3 encodes a histone deacetylase (7). Covalent modifications of histones, including acetylation and deacetylation, are implicated in transcriptional regulation in yeast and other eukaryotes, and have been reviewed in 9 and 10. Rpd3p is found in a complex that also contains Sin3p and Sap30p and is involved in transcriptional silencing (11, 12, 13). A second histone deactylase, Hda1p, shows sequence similarity to Rpd3p but is found in a complex distinct from the complex containing Rpd3p (7); the two complexes affect transcription of distinct, partially overlapping sets of genes (7, 10). Deletion of RPD3 increases histone acetylation in vivo and increases telomeric repression of transcription (7), and extends life span (14). In diploids, mutations in RPD3 cause defects in mitotic recombination and sporulation (2), and the recombination mutant rec3-1 (15) has been shown to be an allele of RPD3 (2). Three more histone deactylases, Hos1p, Hos2p, and Hos3p, have been identified in yeast; they share sequence similarity with Rpd3p and Hda1p but are less well characterized (7).

Last updated: 2000-01-06

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RPD3]

1) Vidal M, et al.  (1990) Direct selection for mutants with increased K+ transport in Saccharomyces cerevisiae. Genetics 125(2):313-20
2) Dora EG, et al.  (1999) RPD3 (REC3) mutations affect mitotic recombination in Saccharomyces cerevisiae. Curr Genet 35(2):68-76
3) Stillman DJ, et al.  (1994) Epistasis analysis of suppressor mutations that allow HO expression in the absence of the yeast SW15 transcriptional activator. Genetics 136(3):781-8
4) Sussel L, et al.  (1993) Epigenetic switching of transcriptional states: cis- and trans-acting factors affecting establishment of silencing at the HMR locus in Saccharomyces cerevisiae. Mol Cell Biol 13(7):3919-28
5) Sussel L, et al.  (1995) Suppressors of defective silencing in yeast: effects on transcriptional repression at the HMR locus, cell growth and telomere structure. Genetics 141(3):873-88
6) Meskauskas A, et al.  (2003) Delayed rRNA processing results in significant ribosome biogenesis and functional defects. Mol Cell Biol 23(5):1602-13
7) Rundlett SE, et al.  (1996) HDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription. Proc Natl Acad Sci U S A 93(25):14503-8
8) Nyswaner KM, et al.  (2008) Chromatin-associated genes protect the yeast genome from ty1 insertional mutagenesis. Genetics 178(1):197-214
9) Mizzen C, et al.  (1998) Signaling to chromatin through histone modifications: how clear is the signal? Cold Spring Harb Symp Quant Biol 63:469-81
10) Suka N, et al.  (1998) The regulation of gene activity by histones and the histone deacetylase RPD3. Cold Spring Harb Symp Quant Biol 63():391-9
11) Sun ZW and Hampsey M  (1999) A general requirement for the Sin3-Rpd3 histone deacetylase complex in regulating silencing in Saccharomyces cerevisiae. Genetics 152(3):921-32
12) Vannier D, et al.  (1996) Evidence that the transcriptional regulators SIN3 and RPD3, and a novel gene (SDS3) with similar functions, are involved in transcriptional silencing in S. cerevisiae. Genetics 144(4):1343-53
13) Kasten MM, et al.  (1997) A large protein complex containing the yeast Sin3p and Rpd3p transcriptional regulators. Mol Cell Biol 17(8):4852-8
14) Kim S, et al.  (1999) Modulation of life-span by histone deacetylase genes in Saccharomyces cerevisiae. Mol Biol Cell 10(10):3125-36
15) Esposito MS and Brown JT  (1990) Conditional hyporecombination mutants of three REC genes of Saccharomyces cerevisiae. Curr Genet 17(1):7-12