LEM3/YNL323W Summary Help

LEM3 BASIC INFORMATION

Standard Name LEM3
Systematic Name YNL323W
Alias BRE3 , ROS3
Feature Type ORF, Verified
Description Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane (1, 2, 3)
Name Description Ligand-Effect Modulator 4
GO Annotations All LEM3 GO evidence and references
    View Computational GO annotations for LEM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All LEM3 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions LEM3 All interactions details and references
60 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • PCA: 3
Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 19
  • Phenotypic Suppression: 16
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1
Sequence Information
ChrXIV:31943 to 33187 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1245 31943..33187 1996-07-31 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005267

LEM3 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for LEM3

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for LEM3]

1) Kato U, et al.  (2002) A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae. J Biol Chem 277(40):37855-62
2) Hanson PK, et al.  (2003) Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine. J Biol Chem 278(38):36041-50
3) Lenoir G, et al.  (2009) Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter drs2p. J Biol Chem 284(27):17956-67
4) Sitcheran R, et al.  (2000) A genetic analysis of glucocorticoid receptor signaling. Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae. Genetics 156(3):963-72