LEM3/YNL323W Summary Help

Standard Name LEM3 1
Systematic Name YNL323W
Alias BRE3 2 , ROS3 3
Feature Type ORF, Verified
Description Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p (3, 4, 5, 6)
Name Description Ligand-Effect Modulator 1
Chromosomal Location
ChrXIV:31944 to 33188 | ORF Map | GBrowse
Gene Ontology Annotations All LEM3 GO evidence and references
  View Computational GO annotations for LEM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
235 total interaction(s) for 131 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • PCA: 7
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 80
  • Phenotypic Enhancement: 36
  • Phenotypic Suppression: 17
  • Positive Genetic: 55
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 6
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 414
Molecular Weight (Da) 47,437
Isoelectric Point (pI) 5.77
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIV:31944 to 33188 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1245 31944..33188 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005267
References cited on this page View Complete Literature Guide for LEM3
1) Sitcheran R, et al.  (2000) A genetic analysis of glucocorticoid receptor signaling. Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae. Genetics 156(3):963-72
2) Muren E, et al.  (2001) Identification of yeast deletion strains that are hypersensitive to brefeldin A or monensin, two drugs that affect intracellular transport. Yeast 18(2):163-72
3) Kato U, et al.  (2002) A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae. J Biol Chem 277(40):37855-62
4) Hanson PK, et al.  (2003) Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine. J Biol Chem 278(38):36041-50
5) Lenoir G, et al.  (2009) Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter drs2p. J Biol Chem 284(27):17956-67
6) Hachiro T, et al.  (2013) Phospholipid flippases lem3p-dnf1p and lem3p-dnf2p are involved in the sorting of the tryptophan permease tat2p in yeast. J Biol Chem 288(5):3594-608