PFS2/YNL317W Summary Help

Standard Name PFS2 1
Systematic Name YNL317W
Feature Type ORF, Verified
Description Integral subunit of the pre-mRNA CPF complex; the cleavage and polyadenylation factor (CPF) complex plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex (2, 3)
Name Description Polyadenylation Factor Subunit 1
Chromosomal Location
ChrXIV:40619 to 42016 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PFS2 GO evidence and references
  View Computational GO annotations for PFS2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
reduction of function
Resources
125 total interaction(s) for 33 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 109
  • Affinity Capture-Western: 5
  • Co-purification: 1
  • Reconstituted Complex: 8

Genetic Interactions
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 465
Molecular Weight (Da) 53,143
Isoelectric Point (pI) 8.16
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:40619 to 42016 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1398 40619..42016 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005261
References cited on this page View Complete Literature Guide for PFS2
1) Keller W and Minvielle-Sebastia L  (1997) A comparison of mammalian and yeast pre-mRNA 3'-end processing. Curr Opin Cell Biol 9(3):329-36
2) Ohnacker M, et al.  (2000) The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex. EMBO J 19(1):37-47
3) Vo LT, et al.  (2001) Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA. Mol Cell Biol 21(24):8346-56