CLA4/YNL298W Summary Help

Standard Name CLA4 1
Systematic Name YNL298W
Alias ERC10 1 , 2 , 3
Feature Type ORF, Verified
Description Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7, 8)
Name Description CLn Activity dependant 9
Chromosomal Location
ChrXIV:68915 to 71443 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CLA4 GO evidence and references
  View Computational GO annotations for CLA4
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 15 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
1152 total interaction(s) for 623 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 25
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Biochemical Activity: 37
  • PCA: 3
  • Protein-peptide: 4
  • Protein-RNA: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 18

Genetic Interactions
  • Dosage Growth Defect: 211
  • Dosage Lethality: 7
  • Dosage Rescue: 10
  • Negative Genetic: 425
  • Phenotypic Enhancement: 26
  • Phenotypic Suppression: 9
  • Positive Genetic: 35
  • Synthetic Growth Defect: 106
  • Synthetic Lethality: 206
  • Synthetic Rescue: 12

Resources
Expression Summary
histogram
Resources
Length (a.a.) 842
Molecular Weight (Da) 93,908
Isoelectric Point (pI) 9.81
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:68915 to 71443 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2529 68915..71443 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005242
References cited on this page View Complete Literature Guide for CLA4
1) Cvrckova F, et al.  (1995) Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast. Genes Dev 9(15):1817-30
2) Benton BK, et al.  (1993) Genetic analysis of Cln/Cdc28 regulation of cell morphogenesis in budding yeast. EMBO J 12(13):5267-75
3) Benton BK, et al.  (1997) Cla4p, a Saccharomyces cerevisiae Cdc42p-activated kinase involved in cytokinesis, is activated at mitosis. Mol Cell Biol 17(9):5067-76
4) Caviston JP, et al.  (2003) The role of Cdc42p GTPase-activating proteins in assembly of the septin ring in yeast. Mol Biol Cell 14(10):4051-66
5) Versele M and Thorner J  (2004) Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4. J Cell Biol 164(5):701-15
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Bartholomew CR and Hardy CF  (2009) p21-activated kinases Cla4 and Ste20 regulate vacuole inheritance in Saccharomyces cerevisiae. Eukaryot Cell 8(4):560-72
8) Lin M, et al.  (2009) The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway. Mol Biol Cell 20(22):4826-37
9) Cvrckova F and Nasmyth K  (1993) Yeast G1 cyclins CLN1 and CLN2 and a GAP-like protein have a role in bud formation. EMBO J 12(13):5277-86