RIM21/YNL294C Summary Help

Standard Name RIM21 1
Systematic Name YNL294C
Alias PAL2 2
Feature Type ORF, Verified
Description pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH (1, 2, 3, 4)
Name Description Regulator of IME2 5
Chromosomal Location
ChrXIV:80260 to 78659 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RIM21 GO evidence and references
  View Computational GO annotations for RIM21
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
170 total interaction(s) for 146 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 103
  • Phenotypic Enhancement: 2
  • Positive Genetic: 12
  • Synthetic Growth Defect: 30
  • Synthetic Lethality: 12
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 533
Molecular Weight (Da) 61,532
Isoelectric Point (pI) 6.7
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:80260 to 78659 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1602 80260..78659 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005238
References cited on this page View Complete Literature Guide for RIM21
1) Penalva MA and Arst HN Jr  (2002) Regulation of gene expression by ambient pH in filamentous fungi and yeasts. Microbiol Mol Biol Rev 66(3):426-46, table of contents
2) Treton B, et al.  (2000) Ambient pH signalling in ascomycetous yeasts involves homologues of the Aspergillus nidulans genes palF and paIH. Mol Gen Genet 263(3):505-13
3) Castrejon F, et al.  (2006) The RIM101 pathway contributes to yeast cell wall assembly and its function becomes essential in the absence of mitogen-activated protein kinase Slt2p. Eukaryot Cell 5(3):507-17
4) Obara K, et al.  (2012) Membrane protein Rim21 plays a central role in sensing ambient pH in Saccharomyces cerevisiae. J Biol Chem 287(46):38473-81
5) Su SS and Mitchell AP  (1993) Identification of functionally related genes that stimulate early meiotic gene expression in yeast. Genetics 133(1):67-77