MSB3/YNL293W Summary

MSB3/YNL293W Summary

Standard Name	MSB3 1
Systematic Name	YNL293W
Alias	GYP3
Feature Type	ORF, Verified
Description	Rab GTPase-activating protein; regulates endocytosis via inactivation of Vps21p at endosomes and vacuole fusion via inactivation of Ypt7p at vacuoles; also acts on Ypt52p and Sec4p; required for proper actin organization; localizes to plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; relocalizes from bud neck to cytoplasm upon DNA replication stress; MSB3 has a paralog, MSB4, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7)
Name Description	Multicopy Suppressor of Bud Emergence 1, 3

Chromosomal Location	ChrXIV:80640 to 82541 \| ORF Map \| GBrowse

Gene Ontology Annotations All MSB3 GO evidence and references

	View Computational GO annotations for MSB3
Molecular Function
Manually curated	Rab GTPase activator activity (IDA, IMP)
Biological Process
Manually curated	actin filament organization (IGI, IPI) endocytosis (IMP, IPI) exocytosis (IGI) regulation of GTPase activity (IMP, IPI) regulation of protein localization (IMP, IPI)
Cellular Component
Manually curated	cellular bud neck (IDA) cellular bud tip (IDA) cytosol (IDA) fungal-type vacuole membrane (IDA) incipient cellular bud site (IDA) late endosome (IDA) plasma membrane (IDA) polarisome (IPI)
High-throughput	cellular bud neck (IDA) cellular bud tip (IDA) cytoplasm (IDA)

Mutant phenotypes All MSB3 Phenotype evidence and references

Classical genetics
null	Vps21p distribution: abnormal resistance to diltiazem hydrochloride: decreased resistance to cadmium dichloride: decreased
overexpression	vacuolar morphology: abnormal
reduction of function	Vps21p distribution: abnormal
Large-scale survey
null	glycogen accumulation: increased competitive fitness: decreased haploproficient resistance to streptomycin: decreased resistance to mefloquine: decreased resistance to L-canavanine: increased resistance to cycloheximide: decreased toxin resistance: decreased viable
overexpression	resistance to sirolimus: decreased vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MSB3 Interaction evidence and references

	89 total interaction(s) for 64 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 1 Biochemical Activity: 17 PCA: 2 Reconstituted Complex: 7 Two-hybrid: 5
Genetic Interactions	Dosage Growth Defect: 7 Dosage Lethality: 3 Dosage Rescue: 20 Negative Genetic: 8 Phenotypic Enhancement: 2 Positive Genetic: 1 Synthetic Growth Defect: 4 Synthetic Lethality: 6
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MSB3 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:80640 to 82541 | |

SGD ORF map

Last Update

Coordinates: 2005-11-07 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1902	80640..82541	2005-11-07	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005237

References cited on this page View Complete Literature Guide for MSB3

1)	Iwanejko L, et al. (1999) Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w. Yeast 15(14):1529-39
2)	Bach S, et al. (2000) Co-deletion of the MSB3 and MSB4 coding regions affects bipolar budding and perturbs the organization of the actin cytoskeleton. Yeast 16(11):1015-23
3)	Albert S and Gallwitz D (1999) Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition. J Biol Chem 274(47):33186-9
4)	Gao XD, et al. (2003) The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J Cell Biol 162(4):635-46
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Lachmann J, et al. (2012) The Msb3/Gyp3 GAP controls the activity of the Rab GTPases Vps21 and Ypt7 at endosomes and vacuoles. Mol Biol Cell 23(13):2516-26