MSB3/YNL293W Summary Help

Standard Name MSB3 1
Systematic Name YNL293W
Alias GYP3
Feature Type ORF, Verified
Description Rab GTPase-activating protein; regulates endocytosis via inactivation of Vps21p at endosomes and vacuole fusion via inactivation of Ypt7p at vacuoles; also acts on Ypt52p and Sec4p; required for proper actin organization; also localizes to plasma membrane and sites of polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; similar to the TBC-domain Tre2 oncogene; MSB3 has a paralog, MSB4, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8)
Name Description Multicopy Suppressor of Bud Emergence 1, 3
Chromosomal Location
ChrXIV:80640 to 82541 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MSB3 GO evidence and references
  View Computational GO annotations for MSB3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
overexpression
Resources
88 total interaction(s) for 63 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 17
  • PCA: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 6
  • Dosage Lethality: 3
  • Dosage Rescue: 20
  • Negative Genetic: 8
  • Phenotypic Enhancement: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 633
Molecular Weight (Da) 72,999
Isoelectric Point (pI) 7.59
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:80640 to 82541 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1902 80640..82541 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005237
References cited on this page View Complete Literature Guide for MSB3
1) Iwanejko L, et al.  (1999) Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w. Yeast 15(14):1529-39
2) Bach S, et al.  (2000) Co-deletion of the MSB3 and MSB4 coding regions affects bipolar budding and perturbs the organization of the actin cytoskeleton. Yeast 16(11):1015-23
3) Albert S and Gallwitz D  (1999) Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition. J Biol Chem 274(47):33186-9
4) Gao XD, et al.  (2003) The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J Cell Biol 162(4):635-46
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Bizimungu C and Vandenbol M  (2005) At least two regions of the oncoprotein Tre2 are involved in its lack of GAP activity. Biochem Biophys Res Commun 335(3):883-90
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Lachmann J, et al.  (2012) The Msb3/Gyp3 GAP controls the activity of the Rab GTPases Vps21 and Ypt7 at endosomes and vacuoles. Mol Biol Cell 23(13):2516-26