MID1/YNL291C Summary Help

Standard Name MID1 1
Systematic Name YNL291C
Feature Type ORF, Verified
Description N-glycosylated integral membrane protein of the ER and plasma membrane; functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer (1, 2, 3, 4)
Name Description Mating pheromone-Induced Death 1
Chromosomal Location
ChrXIV:85815 to 84169 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MID1 GO evidence and references
  View Computational GO annotations for MID1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
unspecified
Resources
94 total interaction(s) for 80 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 1
  • Co-fractionation: 3
  • PCA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 49
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 26
  • Synthetic Lethality: 6
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 548
Molecular Weight (Da) 61,574
Isoelectric Point (pI) 4.21
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:85815 to 84169 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1647 85815..84169 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000005235
References cited on this page View Complete Literature Guide for MID1
1) Iida H, et al.  (1994) MID1, a novel Saccharomyces cerevisiae gene encoding a plasma membrane protein, is required for Ca2+ influx and mating. Mol Cell Biol 14(12):8259-71
2) Kanzaki M, et al.  (1999) Molecular identification of a eukaryotic, stretch-activated nonselective cation channel. Science 285(5429):882-6
3) Locke EG, et al.  (2000) A homolog of voltage-gated Ca(2+) channels stimulated by depletion of secretory Ca(2+) in yeast. Mol Cell Biol 20(18):6686-94
4) Yoshimura H, et al.  (2004) Subcellular localization and oligomeric structure of the yeast putative stretch-activated Ca2+ channel component Mid1. Exp Cell Res 293(2):185-95