ERG24/YNL280C Summary Help

Standard Name ERG24 1
Systematic Name YNL280C
Feature Type ORF, Verified
Description C-14 sterol reductase; acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions (1, 2, 3, 4 and see Summary Paragraph)
Name Description ERGosterol biosynthesis 1
Chromosomal Location
ChrXIV:110411 to 109095 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ERG24 GO evidence and references
  View Computational GO annotations for ERG24
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 5 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
overexpression
Resources
43 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1
  • PCA: 12
  • Protein-peptide: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 15
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Lethality: 6
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 438
Molecular Weight (Da) 50,615
Isoelectric Point (pI) 9.4
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:110411 to 109095 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1317 110411..109095 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005224
SUMMARY PARAGRAPH for ERG24

ERG24 encodes C-14 sterol reductase, an enzyme that acts in ergosterol biosynthesis (1, 4, 2, 3). Cells lacking ERG24 accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions, but cannot grow on rich (YPD) medium under aerobic conditions (1, 5, 6, 7). On defined media, or on YPD supplemented with calcium, erg24 mutants can grow aerobically (7, 8). Erg24p is a target of morpholine antifungals, and erg24 mutations confer resistance to these drugs (9, 8). Expression of ERG9, which encodes farnesyl-diphosphate farnesyl transferase, is increased in erg24 mutants (10).

An ERG24 homolog has been cloned in S. pombe (11), and the human lamin B receptor contains a domain similar to sterol reductases, and can complement the erg24 null phenotype (12).

Last updated: 2000-09-01 Contact SGD

References cited on this page View Complete Literature Guide for ERG24
1) Lorenz RT and Parks LW  (1992) Cloning, sequencing, and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae. DNA Cell Biol 11(9):685-92
2) Lees ND, et al.  (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
3) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
4) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Marcireau C, et al.  (1992) Construction and growth properties of a yeast strain defective in sterol 14-reductase. Curr Genet 22(4):267-72
6) Bard M, et al.  (1993) Sterol synthesis and viability of erg11 (cytochrome P450 lanosterol demethylase) mutations in Saccharomyces cerevisiae and Candida albicans. Lipids 28(11):963-7
7) Crowley JH, et al.  (1996) Aerobic isolation of an ERG24 null mutant of Saccharomyces cerevisiae. J Bacteriol 178(10):2991-3
8) Crowley JH, et al.  (1998) A calcium-dependent ergosterol mutant of Saccharomyces cerevisiae. Curr Genet 34(2):93-9
9) Lai MH, et al.  (1994) The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway. Gene 140(1):41-9
10) Kennedy MA, et al.  (1999) Transcriptional regulation of the squalene synthase gene (ERG9) in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 1445(1):110-22
11) Smith S  (1995) Cloning and sequence analysis of an ERG24 homolog from Schizosaccharomyces pombe. Gene 155(1):139-40
12) Silve S, et al.  (1998) Human lamin B receptor exhibits sterol C14-reductase activity in Saccharomyces cerevisiae. Biochim Biophys Acta 1392(2-3):233-44