POL2/YNL262W Summary

POL2/YNL262W Summary

Standard Name	POL2 1
Systematic Name	YNL262W
Alias	DUN2 2
Feature Type	ORF, Verified
Description	Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p (3, 4, 5)
Name Description	POLymerase 1

Chromosomal Location	ChrXIV:148212 to 154880 \| ORF Map \| GBrowse

	Genetic position: -170 cM

Gene Ontology Annotations All POL2 GO evidence and references

	View Computational GO annotations for POL2
Molecular Function
Manually curated	DNA-directed DNA polymerase activity (IDA, IMP) contributes_to double-stranded DNA binding (IDA) double-stranded DNA binding (IDA) single-stranded DNA binding (IDA) single-stranded DNA specific 3'-5' exodeoxyribonuclease activity (IMP)
Biological Process
Manually curated	base-excision repair (IMP) DNA replication proofreading (IMP) DNA synthesis involved in DNA repair (IMP) DNA-dependent DNA replication (IDA) double-strand break repair via nonhomologous end joining (IGI, IMP) error-prone translesion synthesis (IDA) gene conversion (IMP) heterochromatin organization involved in chromatin silencing (IGI, IMP) intra-S DNA damage checkpoint (IGI, IMP, IPI) leading strand elongation (IMP) mitotic DNA replication checkpoint (IMP) mitotic sister chromatid cohesion (IMP) nucleotide-excision repair, DNA gap filling (IMP)
Cellular Component
Manually curated	epsilon DNA polymerase complex (IDA) replication fork (IDA)

Mutant phenotypes All POL2 Phenotype evidence and references

Classical genetics
conditional	cell cycle progression in S phase: arrested heat sensitivity: increased resistance to hydroxyurea: decreased resistance to camptothecin: decreased resistance to methyl methanesulfonate: decreased
reduction of function	mutation frequency: increased
repressible	chromosome/plasmid maintenance: decreased
Large-scale survey
null	inviable toxin resistance: increased
reduction of function	competitive fitness: decreased resistance to hydroxyurea: decreased
repressible	cell cycle progression in S phase: abnormal chromosome/plasmid maintenance: decreased
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All POL2 Interaction evidence and references

	187 total interaction(s) for 74 unique genes/features.
Physical Interactions	Affinity Capture-MS: 38 Affinity Capture-RNA: 2 Affinity Capture-Western: 23 Co-crystal Structure: 1 Co-fractionation: 3 Co-purification: 12 Reconstituted Complex: 4 Two-hybrid: 20
Genetic Interactions	Dosage Rescue: 11 Negative Genetic: 1 Phenotypic Enhancement: 27 Phenotypic Suppression: 8 Positive Genetic: 1 Synthetic Growth Defect: 13 Synthetic Lethality: 22 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All POL2 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:148212 to 154880 | |

SGD ORF map

: -170 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..6669	148212..154880	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005206

References cited on this page View Complete Literature Guide for POL2

1)	Morrison A, et al. (1990) A third essential DNA polymerase in S. cerevisiae. Cell 62(6):1143-51
2)	Navas TA, et al. (1995) DNA polymerase epsilon links the DNA replication machinery to the S phase checkpoint. Cell 80(1):29-39
3)	Burgers PM (1998) Eukaryotic DNA polymerases in DNA replication and DNA repair. Chromosoma 107(4):218-27
4)	Shimizu K, et al. (2002) Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae. J Biol Chem 277(40):37422-9
5)	Lou H, et al. (2008) Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint. Mol Cell 32(1):106-17