LTO1/YNL260C Summary Help

Standard Name LTO1 1
Systematic Name YNL260C
Feature Type ORF, Verified
Description Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species (2, 3, 4, 5, 6, 7)
Name Description required for biogenesis of the Large ribosomal subunit and initiation of Translation in Oxygen 1
Chromosomal Location
ChrXIV:157455 to 156859 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All LTO1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
17 total interaction(s) for 9 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 198
Molecular Weight (Da) 23,328
Isoelectric Point (pI) 10.27
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:157455 to 156859 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..597 157455..156859 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005204
References cited on this page View Complete Literature Guide for LTO1
1) Zhai C, et al.  (2014) The function of ORAOV1/LTO1, a gene that is overexpressed frequently in cancer: essential roles in the function and biogenesis of the ribosome. Oncogene 33(4):484-94
2) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
3) Kanemaki M, et al.  (2003) Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature 423(6941):720-4
4) Hazbun TR, et al.  (2003) Assigning function to yeast proteins by integration of technologies. Mol Cell 12(6):1353-65
5) Ishtar Snoek IS and Yde Steensma H  (2006) Why does Kluyveromyces lactis not grow under anaerobic conditions? Comparison of essential anaerobic genes of Saccharomyces cerevisiae with the Kluyveromyces lactis genome. FEMS Yeast Res 6(3):393-403
6) Perocchi F, et al.  (2006) Assessing Systems Properties of Yeast Mitochondria through an Interaction Map of the Organelle. PLoS Genet 2(10):e170
7) Jin R, et al.  (2008) Large-scale analysis of yeast filamentous growth by systematic gene disruption and overexpression. Mol Biol Cell 19(1):284-96