CWC25/YNL245C Summary Help

Standard Name CWC25 1
Systematic Name YNL245C
Feature Type ORF, Verified
Description Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p (1, 2, 3, 4)
Name Description Complexed With Cef1p 1
Chromosomal Location
ChrXIV:186885 to 186346 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CWC25 GO evidence and references
  View Computational GO annotations for CWC25
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 1 genes
Resources
Large-scale survey
null
reduction of function
Resources
40 total interaction(s) for 39 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 10

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 10
  • Positive Genetic: 3
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 179
Molecular Weight (Da) 20,374
Isoelectric Point (pI) 10.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:186885 to 186346 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..540 186885..186346 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005189
References cited on this page View Complete Literature Guide for CWC25
1) Ohi MD, et al.  (2002) Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol Cell Biol 22(7):2011-24
2) Hazbun TR, et al.  (2003) Assigning function to yeast proteins by integration of technologies. Mol Cell 12(6):1353-65
3) Warkocki Z, et al.  (2009) Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components. Nat Struct Mol Biol 16(12):1237-43
4) Chiu YF, et al.  (2009) Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction. Mol Cell Biol 29(21):5671-8