BNI4/YNL233W Summary Help

BNI4 BASIC INFORMATION

Standard Name BNI4 1
Systematic Name YNL233W
Feature Type ORF, Verified
Description Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p (2 and see Summary Paragraph)
Name Description Bud Neck Involved 1
GO Annotations All BNI4 GO evidence and references
    View Computational GO annotations for BNI4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All BNI4 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions BNI4 All interactions details and references
127 total interaction(s) for 68 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-Western: 4
  • Two-hybrid: 7
Genetic Interactions
  • Dosage Lethality: 5
  • Phenotypic Enhancement: 27
  • Phenotypic Suppression: 12
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 65
Sequence Information
ChrXIV:211923 to 214601 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2679 211923..214601 2005-11-07 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005177

BNI4 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Localization Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for BNI4

SUMMARY PARAGRAPH for BNI4

BNI4 encodes a scaffold protein that tethers chitin synthase III (Chs3p) to the bud neck by interacting with septin neck filaments and with Skt5p, which is a regulatory subunit of chitin synthase III (2). Bni4p is also involved in targeting protein phosphatase 1 (Glc7p) to the bud neck before bud emergence (2). Bni4p is required for the assembly of the chitin ring, and is also involved in septum formation and the maintenance of bud neck integrity (3).

Bni4p localization is regulated during the cell cycle, with the protein located on the exterior of the septin ring before budding and on the mother side of the collar after budding (4). Proper localization of Bni4p requires the actin cytoskeleton, and binding to Glc7p, since cells containing mutant bni4 that fails to associate with Glc7p display mislocalization of Bni4p (2, 4). Bni4 is phosphorylated in a cell cycle-dependent manner, and inactivation of the protein kinase Hsl1p affects Bni4p localization, but there is no evidence that Hsl1p directly phosphorylates Bni4p (2).

bni4 null mutants are viable, but display altered deposition of chitin, and mislocalization of Chs3p, Skt5p, and Glc7p (3, 2).

Last updated: 2005-09-20

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BNI4]

1) DeMarini DJ, et al.  (1997) A septin-based hierarchy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiae cell wall. J Cell Biol 139(1):75-93
2) Kozubowski L, et al.  (2003) A Bni4-Glc7 phosphatase complex that recruits chitin synthase to the site of bud emergence. Mol Biol Cell 14(1):26-39
3) Sanz M, et al.  (2004) Saccharomyces cerevisiae Bni4p directs the formation of the chitin ring and also participates in the correct assembly of the septum structure. Microbiology 150(Pt 10):3229-41
4) Kozubowski L, et al.  (2005) Role of the septin ring in the asymmetric localization of proteins at the mother-bud neck in Saccharomyces cerevisiae. Mol Biol Cell 16(8):3455-66