SSU72/YNL222W Summary Help

Standard Name SSU72 1
Systematic Name YNL222W
Feature Type ORF, Verified
Description Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo (1, 2, 3, 4)
Name Description Suppressor of SUa7, gene 2 1
Chromosomal Location
ChrXIV:229094 to 229714 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SSU72 GO evidence and references
  View Computational GO annotations for SSU72
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
conditional
Large-scale survey
conditional
null
reduction of function
repressible
unspecified
Resources
240 total interaction(s) for 134 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 78
  • Affinity Capture-Western: 11
  • Biochemical Activity: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 96
  • Phenotypic Enhancement: 2
  • Positive Genetic: 10
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 9
  • Synthetic Rescue: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 206
Molecular Weight (Da) 23,469
Isoelectric Point (pI) 5.03
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:229094 to 229714 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..621 229094..229714 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005166
References cited on this page View Complete Literature Guide for SSU72
1) Sun ZW and Hampsey M  (1996) Synthetic enhancement of a TFIIB defect by a mutation in SSU72, an essential yeast gene encoding a novel protein that affects transcription start site selection in vivo. Mol Cell Biol 16(4):1557-66
2) Krishnamurthy S, et al.  (2004) Ssu72 Is an RNA polymerase II CTD phosphatase. Mol Cell 14(3):387-94
3) St-Pierre B, et al.  (2005) Conserved and specific functions of mammalian ssu72. Nucleic Acids Res 33(2):464-77
4) Zhang DW, et al.  (2012) Ssu72 phosphatase-dependent erasure of phospho-Ser7 marks on the RNA polymerase II C-terminal domain is essential for viability and transcription termination. J Biol Chem 287(11):8541-51