MGS1/YNL218W Summary Help

Standard Name MGS1 1
Systematic Name YNL218W
Feature Type ORF, Verified
Description Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress (1, 2, 3, 4)
Name Description Maintenance of Genome Stability 1
Chromosomal Location
ChrXIV:238238 to 240001 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MGS1 GO evidence and references
  View Computational GO annotations for MGS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
101 total interaction(s) for 58 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 15
  • Dosage Rescue: 3
  • Negative Genetic: 13
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 3
  • Positive Genetic: 2
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 8
  • Synthetic Rescue: 13

Resources
Expression Summary
histogram
Resources
Length (a.a.) 587
Molecular Weight (Da) 66,543
Isoelectric Point (pI) 8.47
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:238238 to 240001 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1764 238238..240001 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005162
References cited on this page View Complete Literature Guide for MGS1
1) Hishida T, et al.  (2001) A yeast gene, MGS1, encoding a DNA-dependent AAA(+) ATPase is required to maintain genome stability. Proc Natl Acad Sci U S A 98(15):8283-9
2) Hishida T, et al.  (2002) Saccharomyces cerevisiae MGS1 is essential in strains deficient in the RAD6-dependent DNA damage tolerance pathway. EMBO J 21(8):2019-29
3) Branzei D, et al.  (2002) The product of Saccharomyces cerevisiae WHIP/MGS1, a gene related to replication factor C genes, interacts functionally with DNA polymerase delta. Mol Genet Genomics 268(3):371-86
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76