RAP1/YNL216W Summary Help

RAP1 BASIC INFORMATION

Standard Name RAP1
Systematic Name YNL216W
Alias GRF1 1 , TBA1 , TUF1
Feature Type ORF, Verified
Description DNA-binding protein involved in either activation or repression of transcription, depending on binding site context; also binds telomere sequences and plays a role in telomeric position effect (silencing) and telomere structure (2 and see Summary Paragraph)
Name Description Repressor Activator Protein
GO Annotations All RAP1 GO evidence and references
    View Computational GO annotations for RAP1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs RAP1 Transcription Factor Binding Sites and References
  • CAYCCRTRCA
  • WRMACCCATACAYY
Mutant Phenotype All RAP1 Phenotype details and references
Classical genetics
unspecified
Large-scale survey
null
overexpression
Interactions RAP1 All interactions details and references
118 total interaction(s) for 88 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 11
  • Co-localization: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 19
Genetic Interactions
  • Dosage Lethality: 3
  • Dosage Rescue: 4
  • Phenotypic Enhancement: 30
  • Phenotypic Suppression: 18
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 16
Sequence Information
ChrXIV:241690 to 244173 | ORF Map | GBrowse
Gbrowse
Genetic position: -148 cM
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2484 241690..244173 2005-11-07 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005160

RAP1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for RAP1

NOMENCLATURE CONFLICT NOTE

NameRelevanceDescription
TUF1Nomenclature conflictTUF1 has been used in the literature to refer to both RAP1/YNL216W (Shore, D. and Nasmyth, K. (1987) Cell 51:721; PMID:3315231), which encodes a transcription factor and telomere capping protein, and TUF1/YOR187W (Nagata, S. et al. (1983) Proc. Nat. Acad. Sci. 80:6192; PMID:6353412), which encodes mitochondrial translation elongation factor Tu.

SUMMARY PARAGRAPH for RAP1

RAP1 (Repressor Activator Protein) encodes an essential protein involved in many diverse, some seemingly contradictory, processes in S. cerevisiae, including telomere maintenance, transcriptional silencing (repression) of the silent mating loci HML and HMR, and high level transcriptional activation of genes encoding ribosomal proteins and glycolytic enzymes (3, 4, 5, 6). In these various roles, the underlying function of Rap1p is to bind DNA in a sequence specific manner, often regulating the chromatin structure in the region where it binds (7, 8). Rap1p binds extensively in telomeric regions, where its function is related to both transcriptional silencing and telomere maintenance (9). In its role as a transcription activating factor, the largest group of target genes are those that encode ribosomal proteins (6). In rapidly growing yeast cells, the transcription rate of these genes is extremely high, accounting for about twenty percent of the total mRNA content of the cell (10). Rap1p is known to be required for the transcription of several non-ribosomal protein genes, including HIS4 (11), ENO1 and ENO2 (12), and is implicated in transcriptional regulation of 185 additional genes (6).

Last updated: 2003-01-17

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RAP1]

1) Elledge SJ and Davis RW  (1989) Identification of the DNA damage-responsive element of RNR2 and evidence that four distinct cellular factors bind it. Mol Cell Biol 9(12):5373-86
2) Pina B, et al.  (2003) The different (sur)faces of Rap1p. Mol Genet Genomics 268(6):791-8
3) Shore D and Nasmyth K  (1987) Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements. Cell 51(5):721-32
4) Vignais ML, et al.  (1987) Specific binding of TUF factor to upstream activation sites of yeast ribosomal protein genes. EMBO J 6(5):1451-7
5) Buchman AR, et al.  (1988) Connections between transcriptional activators, silencers, and telomeres as revealed by functional analysis of a yeast DNA-binding protein. Mol Cell Biol 8(12):5086-99
6) Lieb JD, et al.  (2001) Promoter-specific binding of Rap1 revealed by genome-wide maps of protein-DNA association. Nat Genet 28(4):327-34
7) Morse RH  (2000) RAP, RAP, open up! New wrinkles for RAP1 in yeast. Trends Genet 16(2):51-3
8) Konig P, et al.  (1996) The crystal structure of the DNA-binding domain of yeast RAP1 in complex with telomeric DNA. Cell 85(1):125-36
9) Shore D  (1997) Telomere length regulation: getting the measure of chromosome ends. Biol Chem 378(7):591-7
10) Lascaris RF, et al.  (1999) DNA-binding requirements of the yeast protein Rap1p as selected in silico from ribosomal protein gene promoter sequences. Bioinformatics 15(4):267-77
11) Devlin C, et al.  (1991) RAP1 is required for BAS1/BAS2- and GCN4-dependent transcription of the yeast HIS4 gene. Mol Cell Biol 11(7):3642-51
12) Brindle PK, et al.  (1990) Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription. Mol Cell Biol 10(9):4872-85