PSY2/YNL201C Summary Help

Standard Name PSY2 1
Systematic Name YNL201C
Feature Type ORF, Verified
Description Subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form the active complex, Psy4p may provide additional substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 (2, 3, 4, 5, 6)
Name Description Platinum SensitivitY 1
Chromosomal Location
ChrXIV:263203 to 260627 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PSY2 GO evidence and references
  View Computational GO annotations for PSY2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
189 total interaction(s) for 117 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 54
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • Biochemical Activity: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 13

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 76
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 5
  • Positive Genetic: 11
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 4
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 858
Molecular Weight (Da) 98,052
Isoelectric Point (pI) 4.81
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:263203 to 260627 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2577 263203..260627 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005145
References cited on this page View Complete Literature Guide for PSY2
1) Wu HI, et al.  (2004) Genome-wide identification of genes conferring resistance to the anticancer agents cisplatin, oxaliplatin, and mitomycin C. Cancer Res 64(11):3940-8
2) Keogh MC, et al.  (2006) A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature 439(7075):497-501
3) Hastie CJ, et al.  (2006) The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin. FEBS J 273(14):3322-34
4) O'Neill BM, et al.  (2007) Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage. Proc Natl Acad Sci U S A 104(22):9290-5
5) Falk JE, et al.  (2010) A Mec1- and PP4-dependent checkpoint couples centromere pairing to meiotic recombination. Dev Cell 19(4):599-611
6) Omidi K, et al.  (2014) Phosphatase Complex Pph3/Psy2 Is Involved in Regulation of Efficient Non-Homologous End-Joining Pathway in the Yeast Saccharomyces cerevisiae. PLoS One 9(1):e87248