SRP1/YNL189W Summary

SRP1 BASIC INFORMATION

Standard Name	SRP1
Systematic Name	YNL189W
Alias	KAP60 , SCM1
Feature Type	ORF, Verified
Description	Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation (1, 2)
Name Description	Suppressor of rpb1

GO Annotations	All SRP1 GO evidence and references
	View Computational GO annotations for SRP1
Molecular Function
Manually curated	protein transporter activity (IDA)
Biological Process
Manually curated	protein import into nucleus (IMP) protein targeting to membrane (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) colocalizes_with nuclear exosome (RNase complex) (IDA) nucleus (IDA)
High-throughput	nucleus (IDA)

Mutant Phenotype	All SRP1 Phenotype details and references
Classical genetics
conditional	cell cycle progression through the G1/S phase transition: abnormal cold sensitivity: increased heat sensitivity: increased nuclear import: decreased nuclear import: normal resistance to benomyl: decreased resistance to benomyl: normal resistance to hydroxyurea: decreased
unspecified	actin cytoskeleton morphology: abnormal endocytosis: decreased
Large-scale survey
conditional	heat sensitivity: increased
null	filamentous growth: decreased invasive growth: absent inviable

Interactions	SRP1 All interactions details and references
	366 total interaction(s) for 209 unique genes/features.
Physical Interactions	Affinity Capture-MS: 166 Affinity Capture-RNA: 1 Affinity Capture-Western: 26 Biochemical Activity: 5 Co-crystal Structure: 1 Co-localization: 1 Co-purification: 3 Protein-peptide: 2 Reconstituted Complex: 28 Two-hybrid: 84
Genetic Interactions	Dosage Rescue: 5 Phenotypic Enhancement: 30 Phenotypic Suppression: 4 Synthetic Growth Defect: 2 Synthetic Lethality: 7 Synthetic Rescue: 1

Sequence Information

ChrXIV:284261 to 285889 | |

Last Update

Coordinates: 2005-11-07 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1629	284261..285889	2005-11-07	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005133

SRP1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SRP1

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
TIR1	Nomenclature conflict	SRP1 has been used to refer to both SRP1/YNL189W, which encodes a protein involved in nucleocytoplasmic transport, and TIR1/YER011W, a serine-rich protein of the cell wall.

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SRP1]

1)	Tabb MM, et al. (2000) Evidence for separable functions of Srp1p, the yeast homolog of importin alpha (Karyopherin alpha): role for Srp1p and Sts1p in protein degradation. Mol Cell Biol 20(16):6062-73
2)	Enenkel C, et al. (1995) Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes. J Biol Chem 270(28):16499-502

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