SRP1/YNL189W Summary Help

Standard Name SRP1
Systematic Name YNL189W
Alias KAP60 , SCM1
Feature Type ORF, Verified
Description Karyopherin alpha homolog; forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; involved in cotranslational protein degradation; binds ribosome-bound nascent polypeptides; Srp1p and Sts1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation (1, 2, 3)
Name Description Suppressor of rpb1
Chromosomal Location
ChrXIV:284260 to 285888 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SRP1 GO evidence and references
  View Computational GO annotations for SRP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
unspecified
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
418 total interaction(s) for 239 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 193
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 32
  • Biochemical Activity: 5
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 3
  • PCA: 2
  • Protein-peptide: 2
  • Reconstituted Complex: 39
  • Two-hybrid: 85

Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 28
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 2
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 9
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 542
Molecular Weight (Da) 60,441
Isoelectric Point (pI) 4.63
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:284260 to 285888 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1629 284260..285888 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005133
References cited on this page View Complete Literature Guide for SRP1
1) Tabb MM, et al.  (2000) Evidence for separable functions of Srp1p, the yeast homolog of importin alpha (Karyopherin alpha): role for Srp1p and Sts1p in protein degradation. Mol Cell Biol 20(16):6062-73
2) Enenkel C, et al.  (1995) Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes. J Biol Chem 270(28):16499-502
3) Ha SW, et al.  (2014) Nuclear import factor Srp1 and its associated protein Sts1 couple ribosome-bound nascent polypeptides to proteasomes for cotranslational degradation. J Biol Chem 289(5):2701-10