IPI3/YNL182C Summary Help

Standard Name IPI3 1
Systematic Name YNL182C
Feature Type ORF, Verified
Description Component of the Rix1 complex and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene; other members include Rix1p, Ipi1p, and Ipi3p (1, 2, 3)
Name Description Involved in Processing ITS2 1
Chromosomal Location
ChrXIV:297627 to 295960 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All IPI3 GO evidence and references
  View Computational GO annotations for IPI3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
conditional
null
repressible
Large-scale survey
null
overexpression
reduction of function
Resources
117 total interaction(s) for 56 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 66
  • Affinity Capture-Western: 13
  • Biochemical Activity: 4
  • Co-fractionation: 3
  • Co-purification: 2
  • PCA: 2
  • Two-hybrid: 27

Resources
Expression Summary
histogram
Resources
Length (a.a.) 555
Molecular Weight (Da) 61,772
Isoelectric Point (pI) 5.56
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:297627 to 295960 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1668 297627..295960 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005126
References cited on this page View Complete Literature Guide for IPI3
1) Krogan NJ, et al.  (2004) High-definition macromolecular composition of yeast RNA-processing complexes. Mol Cell 13(2):225-39
2) Nissan TA, et al.  (2004) A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits. Mol Cell 15(2):295-301
3) Huo L, et al.  (2012) The Rix1 (Ipi1p-2p-3p) complex is a critical determinant of DNA replication licensing independent of their roles in ribosome biogenesis. Cell Cycle 11(7):1325-39