APC1/YNL172W Summary Help

Standard Name APC1 1
Systematic Name YNL172W
Feature Type ORF, Verified
Description Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress (1, 2, 3, 4, 5)
Name Description Anaphase Promoting Complex subunit 1
Chromosomal Location
ChrXIV:310636 to 315882 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All APC1 GO evidence and references
  View Computational GO annotations for APC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Large-scale survey
null
reduction of function
Resources
107 total interaction(s) for 50 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 55
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 15
  • Biochemical Activity: 3
  • Co-fractionation: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,748
Molecular Weight (Da) 196,161
Isoelectric Point (pI) 5.8
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:310636 to 315882 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..5247 310636..315882 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005116
References cited on this page View Complete Literature Guide for APC1
1) Zachariae W, et al.  (1996) Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae. Science 274(5290):1201-4
2) Zachariae W, et al.  (1998) Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science 279(5354):1216-9
3) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
4) Buschhorn BA, et al.  (2011) Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nat Struct Mol Biol 18(1):6-13
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76