YCK2/YNL154C Summary

YCK2/YNL154C Summary

Standard Name	YCK2
Systematic Name	YNL154C
Feature Type	ORF, Verified
Description	Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description	Yeast Casein Kinase 5

Chromosomal Location	ChrXIV:345276 to 343636 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All YCK2 GO evidence and references

	View Computational GO annotations for YCK2
Molecular Function
Manually curated	protein serine/threonine kinase activity (IDA)
High-throughput	protein kinase activity (IDA)
Biological Process
Manually curated	cell morphogenesis (IGI) cytokinesis (IGI) endocytosis (IGI) protein phosphorylation (IMP, ISS) response to glucose stimulus (IMP)
High-throughput	protein phosphorylation (IDA)
Cellular Component
Manually curated	cellular bud neck (IDA) mating projection (IDA) plasma membrane (IDA)
High-throughput	plasma membrane (IDA)

Mutant phenotypes All YCK2 Phenotype evidence and references

Classical genetics
null	toxin resistance: decreased
unspecified	resistance to papulacandin B: decreased
Large-scale survey
null	competitive fitness: decreased haploinsufficient resistance to sirolimus: decreased vacuolar morphology: abnormal viable
overexpression	filamentous growth: increased
unspecified	resistance to Calcofluor White: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All YCK2 Interaction evidence and references

	423 total interaction(s) for 385 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-RNA: 1 Affinity Capture-Western: 1 Biochemical Activity: 224 Co-fractionation: 1 Co-localization: 1 Reconstituted Complex: 10 Two-hybrid: 8
Genetic Interactions	Dosage Growth Defect: 2 Dosage Rescue: 1 Negative Genetic: 138 Phenotypic Enhancement: 4 Phenotypic Suppression: 1 Positive Genetic: 7 Synthetic Growth Defect: 2 Synthetic Lethality: 9 Synthetic Rescue: 6
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All YCK2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:345276 to 343636 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1641	345276..343636	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005098

References cited on this page View Complete Literature Guide for YCK2

1)	Marchal C, et al. (2000) Casein kinase I-dependent phosphorylation within a PEST sequence and ubiquitination at nearby lysines signal endocytosis of yeast uracil permease. J Biol Chem 275(31):23608-14
2)	Hicke L, et al. (1998) Cytoplasmic tail phosphorylation of the alpha-factor receptor is required for its ubiquitination and internalization. J Cell Biol 141(2):349-58
3)	Wang PC, et al. (1992) Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1. Mol Biol Cell 3(3):275-86
4)	Robinson LC, et al. (1993) Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesis. Mol Cell Biol 13(5):2870-81
5)	Robinson LC, et al. (1992) Yeast casein kinase I homologues: an essential gene pair. Proc Natl Acad Sci U S A 89(1):28-32
6)	Robinson LC, et al. (1999) The Yck2 yeast casein kinase 1 isoform shows cell cycle-specific localization to sites of polarized growth and is required for proper septin organization. Mol Biol Cell 10(4):1077-92
7)	Feng Y and Davis NG (2000) Akr1p and the type I casein kinases act prior to the ubiquitination step of yeast endocytosis: Akr1p is required for kinase localization to the plasma membrane. Mol Cell Biol 20(14):5350-9
8)	Roth AF, et al. (2002) The yeast DHHC cysteine-rich domain protein Akr1p is a palmitoyl transferase. J Cell Biol 159(1):23-8
9)	Babu P, et al. (2004) Akr1p-dependent palmitoylation of Yck2p yeast casein kinase 1 is necessary and sufficient for plasma membrane targeting. J Biol Chem 279(26):27138-47
10)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61