SRV2/YNL138W Summary

SRV2 BASIC INFORMATION

Standard Name	SRV2 1
Systematic Name	YNL138W
Alias	CAP
Feature Type	ORF, Verified
Description	CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis (1, 2, 3)
Name Description	Suppressor of RasVal19 1

GO Annotations	All SRV2 GO evidence and references
	View Computational GO annotations for SRV2
Molecular Function
Manually curated	adenylate cyclase binding (TAS) cytoskeletal protein binding (TAS)
Biological Process
Manually curated	cytoskeleton organization (TAS) pseudohyphal growth (IMP) Ras protein signal transduction (IMP)
Cellular Component
Manually curated	actin cortical patch (IDA)
High-throughput	mating projection tip (IDA)

Mutant Phenotype	All SRV2 Phenotype details and references
Classical genetics
conditional	heat sensitivity: increased
null	budding pattern: abnormal cell shape: abnormal filamentous growth: abnormal inviable
unspecified	pseudohyphal growth: absent
Large-scale survey
null	bud morphology: abnormal chitin deposition: normal competitive fitness: decreased hyperosmotic stress resistance: decreased lipid particle morphology: abnormal oxidative stress resistance: decreased resistance to mycophenolic acid: decreased resistance to lovastatin: decreased resistance to 5-fluorouracil: decreased resistance to ethanol: decreased resistance to methanol: decreased resistance to propan-1-ol: decreased respiratory growth: absent sporulation: decreased transposable element transposition: increased viable
overexpression	filamentous growth: increased

Interactions	SRV2 All interactions details and references
	92 total interaction(s) for 51 unique genes/features.
Physical Interactions	Affinity Capture-MS: 26 Affinity Capture-Western: 6 Co-fractionation: 3 PCA: 12 Protein-peptide: 2 Reconstituted Complex: 7 Two-hybrid: 15
Genetic Interactions	Dosage Rescue: 2 Phenotypic Enhancement: 2 Phenotypic Suppression: 3 Synthetic Growth Defect: 2 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 10 Synthetic Rescue: 1

Sequence Information

ChrXIV:366743 to 368323 | |

Genetic position: -101 cM

Last Update

Coordinates: 2005-11-07 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1581	366743..368323	2005-11-07	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005082

SRV2 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SRV2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SRV2]

1)	Fedor-Chaiken M, et al. (1990) SRV2, a gene required for RAS activation of adenylate cyclase in yeast. Cell 61(2):329-40
2)	Freeman NL, et al. (1995) An actin monomer binding activity localizes to the carboxyl-terminal half of the Saccharomyces cerevisiae cyclase-associated protein. J Biol Chem 270(10):5680-5
3)	Mattila PK, et al. (2004) A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2/cyclase-associated protein. Mol Biol Cell 15(11):5158-71

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