NAF1/YNL124W Summary Help

Standard Name NAF1 1
Systematic Name YNL124W
Feature Type ORF, Verified
Description RNA-binding protein required for the assembly of box H/ACA snoRNPs; thus required for pre-rRNA processing; forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p (1, 2, 3)
Name Description Nuclear Assembly Factor 1
Chromosomal Location
ChrXIV:392892 to 394370 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NAF1 GO evidence and references
  View Computational GO annotations for NAF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 115 genes
Resources
Classical genetics
null
repressible
Large-scale survey
null
reduction of function
unspecified
Resources
116 total interaction(s) for 95 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 91
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 7
  • Reconstituted Complex: 4
  • Two-hybrid: 11

Genetic Interactions
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 492
Molecular Weight (Da) 54,949
Isoelectric Point (pI) 4.51
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:392892 to 394370 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1479 392892..394370 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005068
References cited on this page View Complete Literature Guide for NAF1
1) Fatica A, et al.  (2002) Naf1 p is a box H/ACA snoRNP assembly factor. RNA 8(12):1502-14
2) Dez C, et al.  (2002) Naf1p, an essential nucleoplasmic factor specifically required for accumulation of box H/ACA small nucleolar RNPs. Mol Cell Biol 22(20):7053-65
3) Yang PK, et al.  (2002) The Shq1p.Naf1p complex is required for box H/ACA small nucleolar ribonucleoprotein particle biogenesis. J Biol Chem 277(47):45235-42