Standard Name	NAF1 1
Systematic Name	YNL124W
Feature Type	ORF, Verified
Description	RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p (1, 2, 3)
Name Description	Nuclear Assembly Factor 1

Chromosomal Location	ChrXIV:392892 to 394370 | ORF Map | GBrowse

	View Computational GO annotations for NAF1
Molecular Function
Manually curated	RNA binding (IPI)
Biological Process
Manually curated	box H/ACA snoRNP assembly (IMP, IPI)
Cellular Component
Manually curated	nucleoplasm (IDA) nucleus (IDA)

Classical genetics
null	inviable
repressible	mature 18S rRNA accumulation: decreased box H/ACA snoRNAs accumulation: decreased 32S rRNA processing intermediate accumulation: decreased 27SA2 rRNA processing intermediate accumulation: decreased aberrant 23S rRNA processing intermediate accumulation: increased 35S pre-rRNA accumulation: increased 20S rRNA processing intermediate accumulation: decreased
Large-scale survey
null	haploinsufficient inviable
reduction of function	competitive fitness: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	115 total interaction(s) for 94 unique genes/features.
Physical Interactions	Affinity Capture-MS: 91 Affinity Capture-RNA: 1 Affinity Capture-Western: 7 Reconstituted Complex: 4 Two-hybrid: 11
Genetic Interactions	Synthetic Lethality: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIV:392892 to 394370 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1479 392892..394370 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005068