MLS1/YNL117W Summary Help

Standard Name MLS1 1
Systematic Name YNL117W
Feature Type ORF, Verified
Description Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA (1, 2, 3)
Name Description MaLate Synthase 1
Chromosomal Location
ChrXIV:406358 to 408022 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MLS1 GO evidence and references
  View Computational GO annotations for MLS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 130 genes
Resources
Pathways
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
32 total interaction(s) for 31 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 3
  • Protein-peptide: 8
  • Reconstituted Complex: 6
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 3
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 554
Molecular Weight (Da) 62,791
Isoelectric Point (pI) 7.18
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:406358 to 408022 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1665 406358..408022 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005061
References cited on this page View Complete Literature Guide for MLS1
1) Hartig A, et al.  (1992) Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S. cerevisiae. Nucleic Acids Res 20(21):5677-86
2) Kunze M, et al.  (2002) Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium. Eur J Biochem 269(3):915-22
3) Branduardi P, et al.  (2013) A novel pathway to produce butanol and isobutanol in Saccharomyces cerevisiae. Biotechnol Biofuels 6(1):68