Standard Name	DMA2 1
Systematic Name	YNL116W
Alias	CHF2 2
Feature Type	ORF, Verified
Description	Ubiquitin-protein ligase (E3); controls septin dynamics and spindle position checkpoint (SPOC) with ligase Dma1p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8, similar to human Chfr; contains FHA and RING finger domains; DMA2 has a paralog, DMA1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)
Name Description	Defective in Mitotic Arrest 1

Chromosomal Location	ChrXIV:408341 to 409909 | ORF Map | GBrowse

	View Computational GO annotations for DMA2
Molecular Function
Manually curated	contributes_to ubiquitin-protein ligase activity (IDA)
Biological Process
Manually curated	cellular bud neck septin ring organization (IGI) establishment of mitotic spindle orientation (IGI) mitotic spindle orientation checkpoint (IGI) protein autoubiquitination (IDA) protein localization to bud neck (IGI) septin ring assembly (IGI)
Cellular Component
High-throughput	cytoplasm (IDA)

Classical genetics
null	Matalpha1p accumulation: increased viable
overexpression	cytokinesis: decreased
Large-scale survey
null	competitive fitness: increased haploproficient sporulation: normal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	95 total interaction(s) for 71 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 2 Affinity Capture-Western: 1 Biochemical Activity: 6 PCA: 1 Two-hybrid: 4
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 3 Negative Genetic: 23 Phenotypic Enhancement: 2 Phenotypic Suppression: 6 Positive Genetic: 8 Synthetic Growth Defect: 3 Synthetic Lethality: 17 Synthetic Rescue: 9
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIV:408341 to 409909 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1569 408341..409909 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005060