RAS2/YNL098C Summary Help

RAS2 BASIC INFORMATION

Standard Name RAS2 1
Systematic Name YNL098C
Alias CTN5 , CYR3 , GLC5 , TSL7
Feature Type ORF, Verified
Description GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes (2, 3, 4 and see Summary Paragraph)
Name Description homologous to RAS proto-oncogene
GO Annotations All RAS2 GO evidence and references
    View Computational GO annotations for RAS2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulatory Role
Regulatory modules predicted: stressResponse ( 589 )
Mutant Phenotype All RAS2 Phenotype details and references
Classical genetics
activation
dominant negative
gain of function
null
overexpression
reduction of function
unspecified
Large-scale survey
null
overexpression
Interactions RAS2 All interactions details and references
283 total interaction(s) for 172 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Biochemical Activity: 6
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 3
Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 7
  • Dosage Rescue: 59
  • Phenotypic Enhancement: 41
  • Phenotypic Suppression: 56
  • Synthetic Growth Defect: 18
  • Synthetic Lethality: 31
  • Synthetic Rescue: 40
Sequence Information
ChrXIV:440572 to 439604 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -71.5 cM
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..969 440572..439604 2005-11-07 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005042

RAS2 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Protein Info & Structure
  • Localization Resources
  • Interactions
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for RAS2

SUMMARY PARAGRAPH for RAS2

RAS2 encodes a homolog of the mammalian oncogene RAS and is highly related to the yeast RAS1 gene (3). Ras2p is a small GTP-binding protein localized to the plasma membrane due to modification of its C-terminus with palmitoyl and farnesyl groups (4). Ras2p regulates processes such as sporulation, filamentous growth and the nitrogen starvation response through its effects on yeast adenylate cyclase (encoded by the CYR1 gene). In the activated, GTP-bound form Ras2p directly stimulates the production of cAMP by adenylate cyclase (5). Cdc25p binds to and activates Ras2p by directly stimulating the exchange of GDP for GTP (6). Conversely, the redundant proteins Ira1p and Ira2p inactivate Ras2p by stimulating hydrolysis of GTP to GDP (7).

Last updated: 1999-03-04

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RAS2]

1) Robinson, L. and Tatchell, K.  (1985) Personal Communication, Mortimer Map Edition 9
2) Gimeno CJ, et al.  (1992) Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS. Cell 68(6):1077-90
3) Kataoka T, et al.  (1984) Genetic analysis of yeast RAS1 and RAS2 genes. Cell 37(2):437-45
4) Bhattacharya S, et al.  (1995) Ras membrane targeting is essential for glucose signaling but not for viability in yeast. Proc Natl Acad Sci U S A 92(7):2984-8
5) Broek D, et al.  (1985) Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins. Cell 41(3):763-9
6) Lai CC, et al.  (1993) Influence of guanine nucleotides on complex formation between Ras and CDC25 proteins. Mol Cell Biol 13(3):1345-52
7) Parrini MC, et al.  (1996) Determinants of Ras proteins specifying the sensitivity to yeast Ira2p and human p120-GAP. EMBO J 15(5):1107-11