RAS2/YNL098C Summary Help

Standard Name RAS2 1
Systematic Name YNL098C
Alias CTN5 , CYR3 , GLC5 , TSL7
Feature Type ORF, Verified
Description GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication (2, 3, 4, 5 and see Summary Paragraph)
Name Description homologous to RAS proto-oncogene
Chromosomal Location
ChrXIV:440570 to 439602 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -71.5 cM
Gene Ontology Annotations All RAS2 GO evidence and references
  View Computational GO annotations for RAS2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulatory Role
Regulatory modules predicted: stressResponse (589)
Mutant phenotypes All RAS2 Phenotype evidence and references
Classical genetics
activation
dominant negative
gain of function
null
overexpression
reduction of function
unspecified
Large-scale survey
null
overexpression
Resources
interactions All RAS2 Interaction evidence and references
462 total interaction(s) for 273 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 8
  • Biochemical Activity: 8
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 3
Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Lethality: 7
  • Dosage Rescue: 66
  • Negative Genetic: 137
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 51
  • Positive Genetic: 30
  • Synthetic Growth Defect: 29
  • Synthetic Lethality: 29
  • Synthetic Rescue: 48
Resources
Expression Summary
histogram
Resources
Protein Information All RAS2 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:440570 to 439602 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -71.5 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..969 440570..439602 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005042
SUMMARY PARAGRAPH for RAS2

RAS2 encodes a homolog of the mammalian oncogene RAS and is highly related to the yeast RAS1 gene (3). Ras2p is a small GTP-binding protein localized to the plasma membrane due to modification of its C-terminus with palmitoyl and farnesyl groups (4). Ras2p regulates processes such as sporulation, filamentous growth and the nitrogen starvation response through its effects on yeast adenylate cyclase (encoded by the CYR1 gene). In the activated, GTP-bound form Ras2p directly stimulates the production of cAMP by adenylate cyclase (6). Cdc25p binds to and activates Ras2p by directly stimulating the exchange of GDP for GTP (7). Conversely, the redundant proteins Ira1p and Ira2p inactivate Ras2p by stimulating hydrolysis of GTP to GDP (8).

Last updated: 1999-03-04

References cited on this page View Complete Literature Guide for RAS2
1) Robinson, L. and Tatchell, K.  (1985) Personal Communication, Mortimer Map Edition 9
2) Gimeno CJ, et al.  (1992) Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS. Cell 68(6):1077-90
3) Kataoka T, et al.  (1984) Genetic analysis of yeast RAS1 and RAS2 genes. Cell 37(2):437-45
4) Bhattacharya S, et al.  (1995) Ras membrane targeting is essential for glucose signaling but not for viability in yeast. Proc Natl Acad Sci U S A 92(7):2984-8
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Broek D, et al.  (1985) Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins. Cell 41(3):763-9
7) Lai CC, et al.  (1993) Influence of guanine nucleotides on complex formation between Ras and CDC25 proteins. Mol Cell Biol 13(3):1345-52
8) Parrini MC, et al.  (1996) Determinants of Ras proteins specifying the sensitivity to yeast Ira2p and human p120-GAP. EMBO J 15(5):1107-11