YPT53/YNL093W Summary Help

Standard Name YPT53 1
Systematic Name YNL093W
Feature Type ORF, Verified
Description Stress-induced Rab family GTPase; required for vacuolar protein sorting and endocytosis; involved in ionic stress tolerance; similar to Vps21p and Ypt52p; functional homolog of Vps21p; mammalian Rab5 homolog; YPT53 has a paralog, VPS21, that arose from the whole genome duplication (1, 2, 3, 4 and see Summary Paragraph)
Name Description Yeast Protein Two 1
Chromosomal Location
ChrXIV:449868 to 450530 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All YPT53 GO evidence and references
  View Computational GO annotations for YPT53
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Large-scale survey
null
Resources
54 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Protein-peptide: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 21

Genetic Interactions
  • Dosage Growth Defect: 2
  • Negative Genetic: 10
  • Phenotypic Enhancement: 3
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 220
Molecular Weight (Da) 24,648
Isoelectric Point (pI) 4.99
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:449868 to 450530 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..663 449868..450530 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005037
SUMMARY PARAGRAPH for YPT53

Rab proteins are small Ras-related GTPases that function in multiple stages in membrane traffic. Rabs continuously cycle between the cytosol and membranes. The GDP-bound form of the Rab is complexed with guanine nucleotide dissociation inhibitor(GDI) in the cytosol and are recruited to the membranes with the help of GDF (GDI Displacement Factor) and by their prenylation by geranylgeranyl transferase II (GGTase II). The membrane-bound Rab is activated to the GTP-bound form by the guanine nucleotide exchange factors (GEFs) which then goes on to interact with downstream effectors. The GTPase-activating protein (GAP) stimulates the hydrolysis of GTP to GDP to inactivate the Rab. Eleven Rabs have been identified in yeast and more than 60 in mammalian cells (reviewed in 5, 6, 7).

Last updated: 2010-05-18 Contact SGD

References cited on this page View Complete Literature Guide for YPT53
1) Singer-Kruger B, et al.  (1994) Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast. J Cell Biol 125(2):283-98
2) Lazar T, et al.  (1997) Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? Trends Biochem Sci 22(12):468-72
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Nickerson DP, et al.  (2012) Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3. Traffic 13(10):1411-28
5) Cai H, et al.  (2007) Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell 12(5):671-82
6) Grosshans BL, et al.  (2006) Rabs and their effectors: achieving specificity in membrane traffic. Proc Natl Acad Sci U S A 103(32):11821-7
7) Bialek-Wyrzykowska U, et al.  (2000) Low levels of Ypt protein prenylation cause vesicle polarization defects and thermosensitive growth that can be suppressed by genes involved in cell wall maintenance. Mol Microbiol 35(6):1295-311