MKT1/YNL085W Summary Help

Standard Name MKT1 1
Systematic Name YNL085W
Feature Type ORF, Verified
Description Protein similar to nucleases that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress; localization to P-bodies under ethanol stress differs between strains (1, 2, 3, 4, 5, 6)
Name Description Maintenance of K2 Killer Toxin 7
Chromosomal Location
ChrXIV:467131 to 469623 | ORF Map | GBrowse
Gbrowse
Genetic position: -69 cM
Gene Ontology Annotations All MKT1 GO evidence and references
  View Computational GO annotations for MKT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
reduction of function
unspecified
Large-scale survey
null
unspecified
Resources
86 total interaction(s) for 73 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 43
  • Affinity Capture-RNA: 3
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 16
  • Phenotypic Suppression: 1
  • Positive Genetic: 13
  • Synthetic Growth Defect: 3
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 830
Molecular Weight (Da) 94,494
Isoelectric Point (pI) 5.41
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:467131 to 469623 | ORF Map | GBrowse
SGD ORF map
Genetic position: -69 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2493 467131..469623 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005029
References cited on this page View Complete Literature Guide for MKT1
1) Wickner RB  (1987) MKT1, a nonessential Saccharomyces cerevisiae gene with a temperature-dependent effect on replication of M2 double-stranded RNA. J Bacteriol 169(11):4941-5
2) Tadauchi T, et al.  (2004) Posttranscriptional regulation of HO expression by the Mkt1-Pbp1 complex. Mol Cell Biol 24(9):3670-81
3) Smith EN and Kruglyak L  (2008) Gene-environment interaction in yeast gene expression. PLoS Biol 6(4):e83
4) Dimitrov LN, et al.  (2009) Polymorphisms in multiple genes contribute to the spontaneous mitochondrial genome instability of Saccharomyces cerevisiae S288C strains. Genetics 183(1):365-83
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Lewis JA, et al.  (2014) Genetic Architecture of Ethanol-Responsive Transcriptome Variation in Saccharomyces cerevisiae Strains. Genetics ()
7) Wickner RB  (1980) Plasmids controlled exclusion of the K2 killer double-stranded RNA plasmid of yeast. Cell 21(1):217-26