MLF3/YNL074C Summary Help

Standard Name MLF3 1
Systematic Name YNL074C
Alias YMK1
Feature Type ORF, Verified
Description Serine-rich protein of unknown function; predicted to be palmitoylated; overproduction suppresses growth inhibition caused by exposure to immunosuppressant leflunomide; MLF3 has a paralog, VHS2, that arose from the whole genome duplication (1, 2, 3)
Name Description Multicopy suppressor of LeFlunomide sensitivity 1
Chromosomal Location
ChrXIV:488124 to 486766 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MLF3 GO evidence and references
  View Computational GO annotations for MLF3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
32 total interaction(s) for 26 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 9
  • Protein-RNA: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 8
  • Negative Genetic: 1
  • Synthetic Growth Defect: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 452
Molecular Weight (Da) 49,210
Isoelectric Point (pI) 7.82
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:488124 to 486766 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1359 488124..486766 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005018
References cited on this page View Complete Literature Guide for MLF3
1) Fujimura HA  (1998) Saccharomyces cerevisiae MLF3/YNL074C gene, encoding a serine-rich protein of unknown function, determines the level of resistance to the novel immunosuppressive drug leflunomide. Biochim Biophys Acta 1442(2-3):415-8
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Ren J, et al.  (2008) CSS-Palm 2.0: an updated software for palmitoylation sites prediction. Protein Eng Des Sel 21(11):639-44