MSG5/YNL053W Summary Help

Standard Name MSG5 1
Systematic Name YNL053W
Feature Type ORF, Verified
Description Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p; MSG5 has a paralog, SDP1, that arose from the whole genome duplication (1, 2, 3, 4 and see Summary Paragraph)
Name Description Multicopy Suppressor of GPA1 1
Chromosomal Location
ChrXIV:529942 to 531411 | ORF Map | GBrowse
Gene Ontology Annotations All MSG5 GO evidence and references
  View Computational GO annotations for MSG5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
98 total interaction(s) for 65 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 6
  • Co-localization: 1
  • PCA: 3
  • Protein-RNA: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 9
  • Negative Genetic: 24
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 2
  • Positive Genetic: 18
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 489
Molecular Weight (Da) 54,217
Isoelectric Point (pI) 7.21
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIV:529942 to 531411 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1470 529942..531411 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004998

MSG5 encodes a dual-specificity protein phosphatase (1) involved in the regulation of at least two mitogen-activated protein kinase (MAPK)-mediated pathways (see 5 and 6 for review of MAPKs and their regulation). Msg5p dephosphorylates Fus3p, which inactivates this mating response MAPK (1) and targets it for export from the nucleus (7, 4). Msg5p also dephosphorylates and inactivates the cell wall integrity MAPK, Slt2p (also known as Mpk1p) (2), which is activated in response to many growth condition stressors (for review, see 8 and references therein). In addition to down-regulating the activities of both of these MAPKs during desensitization or adaptation to stimuli, Msg5p is also responsible for basal dephosphorylation of Fus3p and Slt2p. Deletion of MSG5 results in induction of many genes normally regulated by the mating or cell integrity pathways, even in the absence of stimuli (9). MSG5 transcription is induced during the pheromone response (1), but not under conditions that stimulate the cell integrity pathway (2 and references therein).

Msg5p and Slt2p are reciprocally regulated on the protein level. Slt2p is a substrate of Msg5p, and dephosphorylation by Msg5p decreases Slt2p activity. Conversely, Msg5p is a substrate for phosphorylation by Slt2p. Phosphorylation of Msg5p is thought to decrease its interaction with Slt2p, thereby reducing the ability of Msg5p to inactivate Slt2p (2).

Msg5p is present in both a short (445 amino acids) and a long (489 amino acids) form. Both isoforms are produced from the same gene via alternative translational initiation sites (2). Although both forms are equally active on Slt2p (2), the long form is more efficient at Fus3p inactivation (9). For technical reasons, SGD sequence annotation currently includes only the longer form of MSG5.

Last updated: 2010-03-08 Contact SGD

References cited on this page View Complete Literature Guide for MSG5
1) Doi K, et al.  (1994) MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae. EMBO J 13(1):61-70
2) Flandez M, et al.  (2004) Reciprocal regulation between Slt2 MAPK and isoforms of Msg5 dual-specificity protein phosphatase modulates the yeast cell integrity pathway. J Biol Chem 279(12):11027-34
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Blackwell E, et al.  (2007) The pheromone-induced nuclear accumulation of the Fus3 MAPK in yeast depends on its phosphorylation state and on Dig1 and Dig2. BMC Cell Biol 8:44
5) Chen RE and Thorner J  (2007) Function and regulation in MAPK signaling pathways: lessons learned from the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 1773(8):1311-40
6) Martin H, et al.  (2005) Protein phosphatases in MAPK signalling: we keep learning from yeast. Mol Microbiol 58(1):6-16
7) Blackwell E, et al.  (2003) Effect of the pheromone-responsive G(alpha) and phosphatase proteins of Saccharomyces cerevisiae on the subcellular localization of the Fus3 mitogen-activated protein kinase. Mol Cell Biol 23(4):1135-50
8) Fuchs BB and Mylonakis E  (2009) Our paths might cross: the role of the fungal cell wall integrity pathway in stress response and cross talk with other stress response pathways. Eukaryot Cell 8(11):1616-25
9) Marin MJ, et al.  (2009) Different modulation of the outputs of yeast MAPK-mediated pathways by distinct stimuli and isoforms of the dual-specificity phosphatase Msg5. Mol Genet Genomics 281(3):345-59