SLM2/YNL047C Summary Help

Standard Name SLM2 1
Systematic Name YNL047C
Alias LIT1
Feature Type ORF, Verified
Description Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description Synthetic Lethal with Mss4 1
Chromosomal Location
ChrXIV:541881 to 539911 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SLM2 GO evidence and references
  View Computational GO annotations for SLM2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
72 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 8
  • Negative Genetic: 17
  • Phenotypic Enhancement: 1
  • Positive Genetic: 4
  • Synthetic Lethality: 5
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 656
Molecular Weight (Da) 74,785
Isoelectric Point (pI) 9.22
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:541881 to 539911 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1971 541881..539911 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004992
SUMMARY PARAGRAPH for SLM2

SLM1 and its homolog SLM2 encode phosphatidylinositol-4,5-bisphosphate (PI4,5P2) binding proteins that are downstream effectors of the TOR complex 2 (TORC2; 1, 2) TORC2 is involved in regulating actin cytoskeleton polarization during cell cycle progression, cell wall integrity, and nutrient receptor endocytosis (4, 5). In addition to Avo2p, TORC2 contains Avo1p, Tsc11p, Lst8p, Bit61p, and Tor2p (6, 7, 2). Slm1p and Slm2p associate with TORC2 through direct physical interaction with Avo2p and Bit61p; this interaction also serves to stabilize localization of Slm1p and Slm2p at the plasma membrane (2). This localization is also dependent on the C-terminal pleckstrin homology domain found in Slm1p and Slm2p, phosphorylation of each protein by Tor2p, and the presence of PI4,5P2 (1, 2). SLM1 and SLM2 are essential for growth, and cells containing a slm1slm2 double mutation exhibit depolarization of the actin cytoskeleton and undergo eventual cell lysis (1, 2). Overexpression of Slm1p or Slm2p is able to rescue loss of Tsc11p function (8).

Last updated: 2005-11-08 Contact SGD

References cited on this page View Complete Literature Guide for SLM2
1) Audhya A, et al.  (2004) Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton. EMBO J 23(19):3747-57
2) Fadri M, et al.  (2005) The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2. Mol Biol Cell 16(4):1883-900
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Schmidt A, et al.  (1996) TOR2 is required for organization of the actin cytoskeleton in yeast. Proc Natl Acad Sci U S A 93(24):13780-5
5) deHart AK, et al.  (2003) Receptor internalization in yeast requires the Tor2-Rho1 signaling pathway. Mol Biol Cell 14(11):4676-84
6) Loewith R, et al.  (2002) Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell 10(3):457-68
7) Reinke A, et al.  (2004) TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem 279(15):14752-62
8) Ho HL, et al.  (2005) Saccharomyces cerevisiaeTSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex. Curr Genet 47(5):273-88