FET4/YMR319C Summary Help

Standard Name FET4 1
Systematic Name YMR319C
Feature Type ORF, Verified
Description Low-affinity Fe(II) transporter of the plasma membrane (1, 2 and see Summary Paragraph)
Name Description FErrous Transport 1
Chromosomal Location
ChrXIII:914538 to 912880 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All FET4 GO evidence and references
  View Computational GO annotations for FET4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Classical genetics
Large-scale survey
55 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • Co-fractionation: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 21
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 552
Molecular Weight (Da) 62,791
Isoelectric Point (pI) 5.26
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:914538 to 912880 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1659 914538..912880 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004938

Although originally identified as a low-affinity iron(II) permease (1, 3), Fet4p has since been shown to import several other transition metal ions, including copper (2, 4) and zinc (5). Copper, cobalt, and cadmium inhibit Fet4p (1, 2). Fet4p is an integral protein of the plasma membrane (3, 6). FET4 is not essential, not even in an fet3 background (1). Overexpression of FET4 improves growth under alkaline conditions (7).

Transcription of FET4 is induced by Aft1p in response to low levels of iron (3, 5, 8) or by Zap1p in response to low zinc (5, 9), but not in response to low copper (5). When the high-affinity iron permease component Fet3p is deleted, FET4 is induced by the addition of copper, zinc, cobalt, or manganese (10). It is also induced under anaerobic conditions (8, 11, 12) and repressed by Rox1p in aerobic conditions (5, 8). Rox1p attenuates the activation of FET4 by Aft1p or Zap1p (5).

Last updated: 2005-08-08 Contact SGD

References cited on this page View Complete Literature Guide for FET4
1) Dix DR, et al.  (1994) The FET4 gene encodes the low affinity Fe(II) transport protein of Saccharomyces cerevisiae. J Biol Chem 269(42):26092-9
2) Hassett R, et al.  (2000) The Fe(II) permease Fet4p functions as a low affinity copper transporter and supports normal copper trafficking in Saccharomyces cerevisiae. Biochem J 351 Pt 2():477-84
3) Dix D, et al.  (1997) Characterization of the FET4 protein of yeast. Evidence for a direct role in the transport of iron. J Biol Chem 272(18):11770-7
4) Portnoy ME, et al.  (2001) Metal transporters that contribute copper to metallochaperones in Saccharomyces cerevisiae. Mol Genet Genomics 265(5):873-82
5) Waters BM and Eide DJ  (2002) Combinatorial control of yeast FET4 gene expression by iron, zinc, and oxygen. J Biol Chem 277(37):33749-57
6) Navarre C, et al.  (2002) Subproteomics: identification of plasma membrane proteins from the yeast Saccharomyces cerevisiae. Proteomics 2(12):1706-14
7) Serrano R, et al.  (2004) Copper and iron are the limiting factors for growth of the yeast Saccharomyces cerevisiae in an alkaline environment. J Biol Chem 279(19):19698-704
8) Jensen LT and Culotta VC  (2002) Regulation of Saccharomyces cerevisiae FET4 by oxygen and iron. J Mol Biol 318(2):251-60
9) Lyons TJ, et al.  (2000) Genome-wide characterization of the Zap1p zinc-responsive regulon in yeast. Proc Natl Acad Sci U S A 97(14):7957-62
10) Li L and Kaplan J  (1998) Defects in the yeast high affinity iron transport system result in increased metal sensitivity because of the increased expression of transporters with a broad transition metal specificity. J Biol Chem 273(35):22181-7
11) Ter Linde JJ, et al.  (1999) Genome-wide transcriptional analysis of aerobic and anaerobic chemostat cultures of Saccharomyces cerevisiae. J Bacteriol 181(24):7409-13
12) Piper MD, et al.  (2002) Reproducibility of oligonucleotide microarray transcriptome analyses. An interlaboratory comparison using chemostat cultures of Saccharomyces cerevisiae. J Biol Chem 277(40):37001-8