GLC8/YMR311C Summary Help

Standard Name GLC8 1
Systematic Name YMR311C
Feature Type ORF, Verified
Description Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress (2, 3, 4 and see Summary Paragraph)
Name Description GLyCogen 1
Chromosomal Location
ChrXIII:897603 to 896914 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All GLC8 GO evidence and references
  View Computational GO annotations for GLC8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 3 genes
Classical genetics
reduction of function
Large-scale survey
67 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 1
  • Biochemical Activity: 4
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 6
  • Negative Genetic: 12
  • Phenotypic Enhancement: 1
  • Positive Genetic: 8
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 8
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 229
Molecular Weight (Da) 26,649
Isoelectric Point (pI) 4.4
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:897603 to 896914 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..690 897603..896914 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004928

GLC8 encodes a regulatory subunit of protein phosphatase 1 (2). Glc7p is the catalytic subunit of protein phosphatase 1, and interacts with a collection of noncatalytic regulatory subunits that modulate its subcellular localization, substrate specificity, and enzyme activity (5). Under normal conditions, Glc8p activates Glc7p, but when Glc8p is overproduced, it may also inhibit Glc7p function (6). Glc8p is required for increased Glc7p activity during stationary phase (2), and is involved in chromosome segregation (6), and to a lesser degree in glycogen accumulation (5). GLC8 is glucose repressed, and Glc8p is activated upon phosphorylation by Pho85p complexed with one of four cyclins (Pcl6p, Pcl7p, Pcl8p, or Pcl10p) (3).

Deletion of GLC8 is not lethal, but does result in a 20% reduction in glycogen accumulation compared to wild type (5). Glc8p exhibits similarity to mammalian phosphatase inhibitor-2 proteins (3).

Last updated: 2005-09-20 Contact SGD

References cited on this page View Complete Literature Guide for GLC8
1) Cannon JF, et al.  (1994) Characterization of glycogen-deficient glc mutants of Saccharomyces cerevisiae. Genetics 136(2):485-503
2) Nigavekar SS, et al.  (2002) Glc8 is a glucose-repressible activator of Glc7 protein phosphatase-1. Arch Biochem Biophys 404(1):71-9
3) Tan YS, et al.  (2003) Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo. J Biol Chem 278(1):147-53
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Ramaswamy NT, et al.  (1998) Regulation of yeast glycogen metabolism and sporulation by Glc7p protein phosphatase. Genetics 149(1):57-72
6) Tung HY, et al.  (1995) Regulation of chromosome segregation by Glc8p, a structural homolog of mammalian inhibitor 2 that functions as both an activator and an inhibitor of yeast protein phosphatase 1. Mol Cell Biol 15(11):6064-74