PSE1/YMR308C Summary Help

Standard Name PSE1
Systematic Name YMR308C
Alias KAP121
Feature Type ORF, Verified
Description Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p (1, 2, 3, 4, 5, 6)
Name Description Protein Secretion Enhancer
Chromosomal Location
ChrXIII:892221 to 888952 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PSE1 GO evidence and references
  View Computational GO annotations for PSE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
overexpression
repressible
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
unspecified
Resources
269 total interaction(s) for 151 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 103
  • Affinity Capture-Western: 34
  • Co-crystal Structure: 3
  • Co-fractionation: 1
  • Co-localization: 2
  • Co-purification: 1
  • FRET: 8
  • Protein-peptide: 2
  • Reconstituted Complex: 45
  • Two-hybrid: 23

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 21
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 11
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,089
Molecular Weight (Da) 121,029
Isoelectric Point (pI) 4.45
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:892221 to 888952 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3270 892221..888952 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004925
References cited on this page View Complete Literature Guide for PSE1
1) Seedorf M and Silver PA  (1997) Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc Natl Acad Sci U S A 94(16):8590-5
2) Marelli M, et al.  (1998) Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p. J Cell Biol 143(7):1813-30
3) Isoyama T, et al.  (2001) Nuclear import of the yeast AP-1-like transcription factor Yap1p is mediated by transport receptor Pse1p, and this import step is not affected by oxidative stress. J Biol Chem 276(24):21863-9
4) Leslie DM, et al.  (2002) Kap121p-mediated nuclear import is required for mating and cellular differentiation in yeast. Mol Cell Biol 22(8):2544-55
5) Delahodde A, et al.  (2001) Pse1/Kap121-dependent nuclear localization of the major yeast multidrug resistance (MDR) transcription factor Pdr1. Mol Microbiol 39(2):304-12
6) Ueta R, et al.  (2003) Pse1p mediates the nuclear import of the iron-responsive transcription factor Aft1p in Saccharomyces cerevisiae. J Biol Chem 278(50):50120-7