RKR1/YMR247C Summary Help

Standard Name RKR1 1
Systematic Name YMR247C
Alias LTN1 2
Feature Type ORF, Verified
Description RING domain E3 ubiquitin ligase; involved in ubiquitin-mediated degradation of non-stop proteins; component of ribosome-bound RQC (ribosome quality control) complex required for degradation of polypeptides arising from stalled translation; degrades products of mRNAs lacking a termination codon regardless of a poly(A) tail; functional connections to chromatin modification; homolog of mouse Listerin, mutations in which reported to cause neurodegeneration (1, 2, 3, 4, 5)
Name Description RING domain mutant Killed by rtf1 deletion 1
Chromosomal Location
ChrXIII:768039 to 763351 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All RKR1 GO evidence and references
  View Computational GO annotations for RKR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
48 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Co-fractionation: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 1
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 22
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 1,562
Molecular Weight (Da) 180,184
Isoelectric Point (pI) 5.24
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:768039 to 763351 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4689 768039..763351 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004861
References cited on this page View Complete Literature Guide for RKR1
1) Braun MA, et al.  (2007) Identification of Rkr1, a nuclear RING domain protein with functional connections to chromatin modification in Saccharomyces cerevisiae. Mol Cell Biol 27(8):2800-11
2) Bengtson MH and Joazeiro CA  (2010) Role of a ribosome-associated E3 ubiquitin ligase in protein quality control. Nature 467(7314):470-3
3) Fleischer TC, et al.  (2006) Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes. Genes Dev 20(10):1294-307
4) Brandman O, et al.  (2012) A ribosome-bound quality control complex triggers degradation of nascent peptides and signals translation stress. Cell 151(5):1042-54
5) Matsuda R, et al.  (2014) Protein quality control systems associated with no-go and nonstop mRNA surveillance in yeast. Genes Cells 19(1):1-12