RNT1/YMR239C Summary

RNT1 BASIC INFORMATION

Standard Name	RNT1 1
Systematic Name	YMR239C
Feature Type	ORF, Verified
Description	RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end; involved in polyadenylation-independent transcription termination (1, 2, 3, 4)
Name Description	RNase Three 1

GO Annotations	All RNT1 GO evidence and references
	View Computational GO annotations for RNT1
Molecular Function
Manually curated	ribonuclease III activity (IDA)
Biological Process
Manually curated	chromatin organization (IMP) rRNA processing (IMP) rRNA transcription (IMP) termination of RNA polymerase II transcription, poly(A)-independent (IDA, IMP, IPI) U1 snRNA 3'-end processing (IMP) U4 snRNA 3'-end processing (IMP) U5 snRNA 3'-end processing (IMP)
Cellular Component
Manually curated	nucleolus (IDA) nucleoplasm (IDA)

Mutant Phenotype	All RNT1 Phenotype details and references
Classical genetics
null	3'-extended forms of snRNA accumulation: decreased rate 3'-extended forms of polyadenylated U3 snoRNA accumulation: increased 3'-extended forms of polyadenylated U4 snRNA accumulation: increased vegetative growth: decreased
Large-scale survey
null	inviable vegetative growth: normal
overexpression	vegetative growth: decreased

Interactions	RNT1 All interactions details and references
	42 total interaction(s) for 32 unique genes/features.
Physical Interactions	Affinity Capture-MS: 12 Affinity Capture-Western: 3 Biochemical Activity: 9 PCA: 1 Protein-RNA: 2 Reconstituted Complex: 3 Two-hybrid: 7
Genetic Interactions	Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Synthetic Growth Defect: 1 Synthetic Lethality: 1

Sequence Information

ChrXIII:749676 to 748261 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1416	749676..748261	1996-07-31	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000004852

RNT1 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for RNT1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RNT1]

1)	Elela SA, et al. (1996) RNase III cleaves eukaryotic preribosomal RNA at a U3 snoRNP-dependent site. Cell 85(1):115-24
2)	Abou Elela S and Ares M Jr (1998) Depletion of yeast RNase III blocks correct U2 3' end formation and results in polyadenylated but functional U2 snRNA. EMBO J 17(13):3738-46
3)	Catala M, et al. (2008) Deletion of Rnt1p alters the proportion of open versus closed rRNA gene repeats in yeast. Mol Cell Biol 28(2):619-29
4)	Ghazal G, et al. (2009) Yeast RNase III triggers polyadenylation-independent transcription termination. Mol Cell 36(1):99-109

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