ERG8/YMR220W Summary

Standard Name	ERG8 1
Systematic Name	YMR220W
Feature Type	ORF, Verified
Description	Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate (2, 3 and see Summary Paragraph)
Name Description	ERGosterol biosynthesis 1

Chromosomal Location	ChrXIII:712316 to 713671 \| ORF Map \| GBrowse

Gene Ontology Annotations All ERG8 GO evidence and references

	View Computational GO annotations for ERG8
Molecular Function
Manually curated	phosphomevalonate kinase activity (IMP)
Biological Process
Manually curated	ergosterol biosynthetic process (IMP) farnesyl diphosphate biosynthetic process, mevalonate pathway (IMP) isopentenyl diphosphate biosynthetic process, mevalonate pathway (IMP) organic acid phosphorylation (IMP)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Pathways	mevalonate pathway superpathway of ergosterol biosynthesis

Mutant phenotypes All ERG8 Phenotype evidence and references

Classical genetics
null	auxotrophy heat sensitivity: increased
repressible	resistance to arsenite(1−): decreased
Large-scale survey
null	inviable starvation resistance: decreased
reduction of function	competitive fitness: decreased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased
repressible	mitochondrial morphology: abnormal
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ERG8 Interaction evidence and references

	62 total interaction(s) for 58 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 3
Genetic Interactions	Negative Genetic: 47 Phenotypic Enhancement: 2 Positive Genetic: 6 Synthetic Lethality: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ERG8 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIII:712316 to 713671 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1356	712316..713671	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004833

SUMMARY PARAGRAPH for ERG8

ERG8 encodes phosphomevalonate kinase, a cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol in yeast, from mevalonate (2, 3, 4). ERG8 is essential; the erg8-1 allele is temperature sensitive and lacks phosphomevalonate kinase activity (1, 2).

Last updated: 2000-08-22

References cited on this page View Complete Literature Guide for ERG8

1)	Karst F and Lacroute F (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77
2)	Tsay YH and Robinson GW (1991) Cloning and characterization of ERG8, an essential gene of Saccharomyces cerevisiae that encodes phosphomevalonate kinase. Mol Cell Biol 11(2):620-31
3)	Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
4)	Stryer L (1995) Biochemistry (4th ed.). New York: W. H. Freeman and Company