RAD14/YMR201C Summary Help

Standard Name RAD14
Systematic Name YMR201C
Feature Type ORF, Verified
Description Protein that recognizes and binds damaged DNA during NER; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein; NER stands for nucleotide excision repair (1, 2 and see Summary Paragraph)
Name Description RADiation sensitive
Chromosomal Location
ChrXIII:667044 to 665845 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RAD14 GO evidence and references
  View Computational GO annotations for RAD14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
205 total interaction(s) for 143 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 10
  • Co-fractionation: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 89
  • Phenotypic Enhancement: 41
  • Phenotypic Suppression: 9
  • Positive Genetic: 16
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 3
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 371
Molecular Weight (Da) 43,038
Isoelectric Point (pI) 7.31
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:667044 to 665845 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..27 667044..667018 2011-02-03 1996-07-31
Intron 28..111 667017..666934 2011-02-03 1996-07-31
CDS 112..1200 666933..665845 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004814
SUMMARY PARAGRAPH for RAD14

In S. cerevisiae, nucleotide excision repair (NER) is mediated by Rad1p, Rad2p, Rad4p, Rad7p, Rad10p, Rad14p, Rad16p, Met18p, the transcription factor TFIIH (composed of Rad3p, Ssl1p, Ssl2p, Tfb1p, Tfb2p, Tfb3p), and the heterotrimeric complex RPA (Rfa1p, Rfa2p, Rfa3p). Together these proteins bind DNA lesions, including UV-induced photoproducts and chemical crosslinks, unwind the surrounding duplex, and make incisions on both sides of the damaged DNA, which releases a fragment of 25-30bp (reviewed in 1, 3).

The various NER proteins assemble into four complexes, NEF1-4 (nucleotide excision repair factors; reviewed in 1). Rad14p, Rad1p, and Rad10p form the complex NEF1 (4). In NEF1, Rad14p and Rad10p form tight interactions with Rad1p but not with each other (4, 5). NEF1 targeting is mediated by Rad14p, which recognizes and binds the damaged DNA (6). Together, Rad1p and Rad10p form a single-strand DNA endonuclease that binds DNA and then nicks the damaged DNA strand on the 5' side of the lesion (7, 8). The Rad1p/Rad10p endonuclease is structure-specific and cleaves 3'-ended single stranded DNA at its junction with the duplex DNA (8).

Rad14p is a zinc metalloprotein and mutations in rad14 result in UV sensitivity (9, 10). RAD14 is the functional homolog of the human gene XPA with the two proteins sharing 54% amino acid similarity (10). Mutations in XPA lead to a frequently occurring and severe form of the disorder xeroderma pigmentosum (XP complementation group A; OMIM); clinical symptoms include neurological abnormalities and increased incidence of skin cancer (10).

Last updated: 2006-03-14 Contact SGD

References cited on this page View Complete Literature Guide for RAD14
1) Prakash S and Prakash L  (2000) Nucleotide excision repair in yeast. Mutat Res 451(1-2):13-24
2) de Laat WL, et al.  (1999) Molecular mechanism of nucleotide excision repair. Genes Dev 13(7):768-85
3) Hoeijmakers JH  (1993) Nucleotide excision repair I: from E. coli to yeast. Trends Genet 9(5):173-7
4) Guzder SN, et al.  (1996) Nucleotide excision repair in yeast is mediated by sequential assembly of repair factors and not by a pre-assembled repairosome. J Biol Chem 271(15):8903-10
5) Bailly V, et al.  (1992) Specific complex formation between proteins encoded by the yeast DNA repair and recombination genes RAD1 and RAD10. Proc Natl Acad Sci U S A 89(17):8273-7
6) Guzder SN, et al.  (2006) Complex formation with damage recognition protein Rad14 is essential for Saccharomyces cerevisiae Rad1-Rad10 nuclease to perform its function in nucleotide excision repair in vivo. Mol Cell Biol 26(3):1135-41
7) Tomkinson AE, et al.  (1993) Yeast DNA repair and recombination proteins Rad1 and Rad10 constitute a single-stranded-DNA endonuclease. Nature 362(6423):860-2
8) Bardwell AJ, et al.  (1994) Specific cleavage of model recombination and repair intermediates by the yeast Rad1-Rad10 DNA endonuclease. Science 265(5181):2082-5
9) Guzder SN, et al.  (1993) Yeast DNA-repair gene RAD14 encodes a zinc metalloprotein with affinity for ultraviolet-damaged DNA. Proc Natl Acad Sci U S A 90(12):5433-7
10) Bankmann M, et al.  (1992) Yeast RAD14 and human xeroderma pigmentosum group A DNA-repair genes encode homologous proteins. Nature 355(6360):555-8