Standard Name	ROT1 1
Systematic Name	YMR200W
Feature Type	ORF, Verified
Description	Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation (1, 2, 3, 4, 5)
Name Description	Reversal Of Tor2 lethality 1

Chromosomal Location	ChrXIII:664752 to 665522 | ORF Map | GBrowse

	View Computational GO annotations for ROT1
Molecular Function
Manually curated	unfolded protein binding (IDA, IMP)
Biological Process
Manually curated	'de novo' protein folding (IMP, IPI) budding cell apical bud growth (IGI, IMP) cytokinesis (IMP) endoplasmic reticulum unfolded protein response (IGI) establishment or maintenance of actin cytoskeleton polarity (IMP) fungal-type cell wall biogenesis (IMP) protein folding (IGI) protein N-linked glycosylation (IGI, IPI) protein O-linked mannosylation (IGI)
Cellular Component
Manually curated	endoplasmic reticulum membrane (IDA)
High-throughput	endoplasmic reticulum (IDA)

Classical genetics
null	cell shape: abnormal chitin deposition: increased inviable killer toxin resistance: increased liquid culture appearance: abnormal resistance to sodium dodecyl sulfate: decreased resistance to hygromycin B: decreased viable
reduction of function	growth in exponential phase: decreased rate resistance to tunicamycin: decreased vegetative growth: decreased
Large-scale survey
conditional	heat sensitivity: increased
null	haploinsufficient inviable resistance to neothyonidioside: decreased
reduction of function	competitive fitness: increased resistance to methyl methanesulfonate: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	107 total interaction(s) for 62 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 2 Affinity Capture-Western: 5 Co-fractionation: 1 Reconstituted Complex: 1
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 14 Negative Genetic: 56 Phenotypic Enhancement: 1 Phenotypic Suppression: 2 Positive Genetic: 1 Synthetic Growth Defect: 11 Synthetic Lethality: 7 Synthetic Rescue: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIII:664752 to 665522 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..771 664752..665522 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain CLIB382 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004813