ROT1/YMR200W Summary Help

Standard Name ROT1 1
Systematic Name YMR200W
Feature Type ORF, Verified
Description Molecular chaperone involved in protein folding in ER; mutation causes defects in cell wall synthesis and lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation; transmembrane helix Ser250 is essential for Rot1p to interact with other membrane components and exert its functional role, avoiding exposure of Ser H-bonding group at lipid-exposed surface (1, 2, 3, 4, 5, 6)
Name Description Reversal Of Tor2 lethality 1
Chromosomal Location
ChrXIII:664752 to 665522 | ORF Map | GBrowse
Gene Ontology Annotations All ROT1 GO evidence and references
  View Computational GO annotations for ROT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
125 total interaction(s) for 74 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 5
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 14
  • Negative Genetic: 72
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 7
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 256
Molecular Weight (Da) 28,908
Isoelectric Point (pI) 5.41
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:664752 to 665522 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..771 664752..665522 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004813
References cited on this page View Complete Literature Guide for ROT1
1) Bickle M, et al.  (1998) Cell wall integrity modulates RHO1 activity via the exchange factor ROM2. EMBO J 17(8):2235-45
2) Takeuchi M, et al.  (2006) Saccharomyces cerevisiae Rot1p is an ER-localized membrane protein that may function with BiP/Kar2p in protein folding. J Biochem 139(3):597-605
3) Takeuchi M, et al.  (2006) Causal links between protein folding in the ER and events along the secretory pathway. Autophagy 2(4):323-4
4) Takeuchi M, et al.  (2008) Saccharomyces cerevisiae Rot1 Is an Essential Molecular Chaperone in the Endoplasmic Reticulum. Mol Biol Cell 19(8):3514-25
5) Pasikowska M, et al.  (2012) The essential endoplasmic reticulum chaperone Rot1 is required for protein N- and O-glycosylation in yeast. Glycobiology 22(7):939-47
6) Martinez-Garay CA, et al.  (2014) A transmembrane serine residue in the Rot1 protein is essential for yeast cell viability. Biochem J 458(2):239-49