CIK1/YMR198W Summary Help

Standard Name CIK1 1
Systematic Name YMR198W
Feature Type ORF, Verified
Description Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Chromosome Instability and Karyogamy 1
Chromosomal Location
ChrXIII:659745 to 661529 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CIK1 GO evidence and references
  View Computational GO annotations for CIK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
765 total interaction(s) for 456 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-Western: 3
  • Co-crystal Structure: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 31

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 1
  • Dosage Rescue: 7
  • Negative Genetic: 523
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Positive Genetic: 103
  • Synthetic Growth Defect: 30
  • Synthetic Lethality: 53
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 594
Molecular Weight (Da) 69,069
Isoelectric Point (pI) 6.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:659745 to 661529 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1785 659745..661529 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004811
References cited on this page View Complete Literature Guide for CIK1
1) Page BD and Snyder M  (1992) CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae. Genes Dev 6(8):1414-29
2) Barrett JG, et al.  (2000) The Kar3p kinesin-related protein forms a novel heterodimeric structure with its associated protein Cik1p. Mol Biol Cell 11(7):2373-85
3) Page BD, et al.  (1994) Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. J Cell Biol 124(4):507-19
4) Manning BD and Snyder M  (2000) Drivers and passengers wanted! the role of kinesin-associated proteins. Trends Cell Biol 10(7):281-9
5) Cottingham FR, et al.  (1999) Novel roles for saccharomyces cerevisiae mitotic spindle motors. J Cell Biol 147(2):335-50
6) Shanks RM, et al.  (2001) The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae. Genetics 159(3):939-51
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Benanti JA, et al.  (2009) Functionally distinct isoforms of Cik1 are differentially regulated by APC/C-mediated proteolysis. Mol Cell 33(5):581-90