PAI3/YMR174C Summary Help

Standard Name PAI3 1
Systematic Name YMR174C
Feature Type ORF, Verified
Description Cytoplasmic proteinase A (Pep4p) inhibitor; dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact (2, 3, 4, 5)
Name Description Proteinase A Inhibitor 4
Gene Product Alias IA3 6
Chromosomal Location
ChrXIII:610365 to 610159 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PAI3 GO evidence and references
  View Computational GO annotations for PAI3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 4 genes
Large-scale survey
11 total interaction(s) for 11 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 2
  • Protein-peptide: 4
  • Reconstituted Complex: 1

Genetic Interactions
  • Negative Genetic: 3
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 68
Molecular Weight (Da) 7,707
Isoelectric Point (pI) 7.68
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:610365 to 610159 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..207 610365..610159 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004786
References cited on this page View Complete Literature Guide for PAI3
1) Schu P and Wolf DH  (1991) The proteinase yscA-inhibitor, IA3, gene. Studies of cytoplasmic proteinase inhibitor deficiency on yeast physiology. FEBS Lett 283(1):78-84
2) Miralles VJ and Serrano R  (1995) A genomic locus in Saccharomyces cerevisiae with four genes up-regulated by osmotic stress. Mol Microbiol 17(4):653-62
3) Phylip LH, et al.  (2001) The potency and specificity of the interaction between the IA3 inhibitor and its target aspartic proteinase from Saccharomyces cerevisiae. J Biol Chem 276(3):2023-30
4) Beck I, et al.  (1980) The intracellular proteinases and their inhibitors in yeast. A mutant with altered regulation of proteinase A inhibitor activity. J Biol Chem 255(10):4821-8
5) Badasso MO, et al.  (2000) Purification, co-crystallization and preliminary X--ray analysis of the natural aspartic proteinase inhibitor IA3 complexed with saccharopepsin from Saccharomyces cerevisiae. Acta Crystallogr D Biol Crystallogr 56(Pt 7):915-7
6) Meussdoerffer F, et al.  (1978) Molecular weight and hydrodynamic properties of a proteinase A inhibitor from baker's yeast. Hoppe Seylers Z Physiol Chem 359(8):993-7