PKR1/YMR123W Summary

PKR1 BASIC INFORMATION

Standard Name	PKR1 1
Systematic Name	YMR123W
Feature Type	ORF, Verified
Description	V-ATPase assembly factor, functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0) (1)
Name Description	Pichia farinosa Killer toxin Resistance 2

GO Annotations	All PKR1 GO evidence and references
	View Computational GO annotations for PKR1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	vacuolar proton-transporting V-type ATPase complex assembly (IGI, IMP)
Cellular Component
Manually curated	integral to endoplasmic reticulum membrane (IDA)

Mutant Phenotype	All PKR1 Phenotype details and references
Classical genetics
null	metal resistance: decreased
Large-scale survey
null	alkaline pH resistance: decreased polyphosphate accumulation: abnormal glutathione excretion: increased chitin deposition: increased competitive fitness: decreased metal resistance: decreased resistance to rapamycin: decreased resistance to wortmannin: normal resistance to L-1,4-dithiothreitol: decreased resistance to calcium dichloride: decreased resistance to doxorubicin: decreased resistance to 1,4-dithiothreitol: decreased resistance to cycloheximide: decreased resistance to fluconazole: decreased respiratory growth: decreased rate respiratory growth: absent toxin resistance: decreased transposable element transposition: decreased utilization of carbon source: decreased utilization of iron source: decreased vegetative growth: decreased viable
overexpression	filamentous growth: increased

Interactions	PKR1 All interactions details and references
	View additional details at BioGRID
	238 total interaction(s) for 157 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 1 PCA: 4
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 177 Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Positive Genetic: 23 Synthetic Growth Defect: 16 Synthetic Lethality: 7

Sequence Information

ChrXIII:513592 to 513960 | |

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..369	513592..513960	1996-07-31	1996-07-31

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000004730

PKR1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for PKR1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PKR1]

1)	Davis-Kaplan SR, et al. (2006) PKR1 encodes an assembly factor for the yeast V-type ATPase. J Biol Chem 281(42):32025-35
2)	Suzuki C and Nikkuni S (1996) Cloning of the Saccharomyces cerevisiae gene whose overexpression gives resistance against Pichia farinosa killer toxin

SGD^tm pages Database Copyright © 1997-2010 The Board of Trustees of Leland Stanford Junior University. Permission to use the information contained in this database was given by the researchers/institutes who contributed or published the information. Users of the database are solely responsible for compliance with any copyright restrictions, including those applying to the author abstracts. Documents from this server are provided "AS-IS" without any warranty, expressed or implied. The SGD project at Stanford University is supported by a Genome Research Resource Grant from the US National Human Genome Research Institute, part of the US National Institutes of Health.