Standard Name	PKR1 1
Systematic Name	YMR123W
Feature Type	ORF, Verified
Description	V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress (1, 2)
Name Description	Pichia farinosa Killer toxin Resistance 3

Chromosomal Location	ChrXIII:513593 to 513961 | ORF Map | GBrowse

	View Computational GO annotations for PKR1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	vacuolar proton-transporting V-type ATPase complex assembly (IGI, IMP)
Cellular Component
Manually curated	integral to endoplasmic reticulum membrane (IDA)

Classical genetics
null	tellurium atom accumulation: increased metal resistance: decreased resistance to tellurite: decreased
Large-scale survey
null	alkaline pH resistance: decreased auxotrophy polyphosphate accumulation: abnormal glutathione excretion: increased chitin deposition: increased competitive fitness: decreased metal resistance: decreased resistance to quinine: decreased resistance to fluconazole: decreased resistance to cycloheximide: decreased resistance to sirolimus: decreased resistance to cycloheximide: increased resistance to 1,4-dithiothreitol and tacrolimus (anhydrous): increased resistance to tacrolimus (anhydrous) and tunicamycin: increased resistance to amitrole: decreased resistance to bleomycin: decreased resistance to caffeine: decreased resistance to fenpropimorph: decreased resistance to tunicamycin: decreased resistance to chloroquine: decreased resistance to calcium dichloride: decreased resistance to wortmannin: normal resistance to ciclopirox olamine: decreased resistance to doxorubicin: decreased resistance to 1,4-dithiothreitol: decreased resistance to L-1,4-dithiothreitol: decreased respiratory growth: decreased rate respiratory growth: absent starvation resistance: decreased stress resistance: increased toxin resistance: decreased transposable element transposition: decreased utilization of carbon source: decreased utilization of iron source: decreased vacuolar morphology: abnormal vegetative growth: decreased viable
overexpression	filamentous growth: increased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	380 total interaction(s) for 224 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-RNA: 1 PCA: 4
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 279 Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Positive Genetic: 54 Synthetic Growth Defect: 26 Synthetic Lethality: 5
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIII:513593 to 513961 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..369 513593..513961 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain CLIB382 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004730