ASC1/YMR116C Summary Help

Standard Name ASC1 1
Systematic Name YMR116C
Alias CPC2 2 , NAD1 3
Feature Type ORF, Verified
Description G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation (1, 2, 4, 5, 6)
Name Description Absence of growth Suppressor of Cyp1 1
Chromosomal Location
ChrXIII:500688 to 499456 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ASC1 GO evidence and references
  View Computational GO annotations for ASC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All ASC1 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
Resources
interactions All ASC1 Interaction evidence and references
304 total interaction(s) for 266 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 81
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 5
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 4
Genetic Interactions
  • Negative Genetic: 143
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 30
  • Synthetic Growth Defect: 19
  • Synthetic Lethality: 6
  • Synthetic Rescue: 3
Resources
Expression Summary
histogram
Resources
Protein Information All ASC1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:500688 to 499456 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
This feature contains embedded feature(s): SNR24
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..537 500688..500152 2011-02-03 1996-07-31
Intron 538..810 500151..499879 2011-02-03 1996-07-31
CDS 811..1233 499878..499456 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004722
References cited on this page View Complete Literature Guide for ASC1
1) Chantrel Y, et al.  (1998) The transcriptional regulator Hap1p (Cyp1p) is essential for anaerobic or heme-deficient growth of Saccharomyces cerevisiae: Genetic and molecular characterization of an extragenic suppressor that encodes a WD repeat protein. Genetics 148(2):559-69
2) Hoffmann B, et al.  (1999) The WD protein Cpc2p is required for repression of Gcn4 protein activity in yeast in the absence of amino-acid starvation. Mol Microbiol 31(3):807-22
3) Kuroha K, et al.  (2010) Receptor for activated C kinase 1 stimulates nascent polypeptide-dependent translation arrest. EMBO Rep 11(12):956-61
4) Gerbasi VR, et al.  (2004) Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression. Mol Cell Biol 24(18):8276-87
5) Zeller CE, et al.  (2007) The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. J Biol Chem 282(34):25168-76
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76