MYO5/YMR109W Summary Help

Standard Name MYO5 1
Systematic Name YMR109W
Feature Type ORF, Verified
Description One of two type I myosin motors; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication (2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description MYOsin 1
Chromosomal Location
ChrXIII:486587 to 490246 | ORF Map | GBrowse
Gene Ontology Annotations All MYO5 GO evidence and references
  View Computational GO annotations for MYO5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
249 total interaction(s) for 117 unique genes/features.
Physical Interactions
  • Affinity Capture-Luminescence: 1
  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 19
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 13
  • Reconstituted Complex: 6
  • Two-hybrid: 77

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 4
  • Negative Genetic: 52
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 13
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 1,219
Molecular Weight (Da) 136,898
Isoelectric Point (pI) 10.09
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:486587 to 490246 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3660 486587..490246 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004715

MYO5 is a non-essential gene that encodes one of two class I myosins found in Saccharomyces cerevisiae (7). Both Myo5p and the other yeast class I myosin, Myo3p, localize to actin cortical patches (1, 8). Cortical patches are found at sites of polarized cell growth, and appear to be involved in endocytosis and cell wall biogenesis (9, 8). Deletion of either MYO3 or MYO5 has little effect on cell growth, but the myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization (10, 1, 2). In a myo3 null background, the temperature sensitive myo5-1 mutant has a defect in the internalization step of endocytosis (2). Physical interaction between Myo5p and calmodulin (Cmd1p) has been detected, and is required for endocytosis (11). Myo5p also interacts physically with verprolin (Vrp1p), and deletion of VRP1 causes delocalization of Myo5p-containing patches (3). Class I myosins play several different roles in many other eukaryotes; see reference 12 for review.

Last updated: 2000-03-24 Contact SGD

References cited on this page View Complete Literature Guide for MYO5
1) Goodson HV, et al.  (1996) Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton. J Cell Biol 133(6):1277-91
2) Geli MI and Riezman H  (1996) Role of type I myosins in receptor-mediated endocytosis in yeast. Science 272(5261):533-5
3) Anderson BL, et al.  (1998) The Src homology domain 3 (SH3) of a yeast type I myosin, Myo5p, binds to verprolin and is required for targeting to sites of actin polarization. J Cell Biol 141(6):1357-70
4) Tanaka K and Matsui Y  (2001) Functions of unconventional myosins in the yeast Saccharomyces cerevisiae. Cell Struct Funct 26(6):671-5
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Sun Y, et al.  (2006) Endocytic internalization in budding yeast requires coordinated actin nucleation and myosin motor activity. Dev Cell 11(1):33-46
7) Brown SS  (1997) Myosins in yeast. Curr Opin Cell Biol 9(1):44-8
8) Pruyne D and Bretscher A  (2000) Polarization of cell growth in yeast. J Cell Sci 113 ( Pt 4):571-85
9) Botstein D, et al.  (1997) "The yeast cytoskeleton." Pp. 1-90 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
10) Goodson HV and Spudich JA  (1995) Identification and molecular characterization of a yeast myosin I. Cell Motil Cytoskeleton 30(1):73-84
11) Geli MI, et al.  (1998) Distinct functions of calmodulin are required for the uptake step of receptor-mediated endocytosis in yeast: the type I myosin Myo5p is one of the calmodulin targets. EMBO J 17(3):635-47
12) Mooseker MS and Cheney RE  (1995) Unconventional myosins. Annu Rev Cell Dev Biol 11():633-75