MYO5/YMR109W Summary Help

MYO5 BASIC INFORMATION

Standard Name MYO5
Systematic Name YMR109W
Feature Type ORF, Verified
Description One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization (1, 2, 3 and see Summary Paragraph)
Name Description MYOsin
GO Annotations All MYO5 GO evidence and references
    View Computational GO annotations for MYO5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All MYO5 Phenotype details and references
Large-scale survey
null
Interactions MYO5 All interactions details and references
164 total interaction(s) for 83 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 16
  • Co-localization: 1
  • PCA: 13
  • Reconstituted Complex: 6
  • Two-hybrid: 77
Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 4
  • Phenotypic Enhancement: 5
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 16
Sequence Information
ChrXIII:486586 to 490245 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3660 486586..490245 1996-07-31 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000004715

MYO5 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Protein Info & Structure
  • Localization Resources
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  • Phenotype Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for MYO5

SUMMARY PARAGRAPH for MYO5

MYO5 is a non-essential gene that encodes one of two class I myosins found in Saccharomyces cerevisiae (4). Both Myo5p and the other yeast class I myosin, Myo3p, localize to actin cortical patches (5, 6) . Cortical patches are found at sites of polarized cell growth, and appear to be involved in endocytosis and cell wall biogenesis (7, 6). Deletion of either MYO3 or MYO5 has little effect on cell growth, but the myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization (8, 5, 1). In a myo3 null background, the temperature sensitive myo5-1 mutant has a defect in the internalization step of endocytosis (1). Physical interaction between Myo5p and calmodulin (Cmd1p) has been detected, and is required for endocytosis (9). Myo5p also interacts physically with verprolin (Vrp1p), and deletion of VRP1 causes delocalization of Myo5p-containing patches (2). Class I myosins play several different roles in many other eukaryotes; see reference 10 for review.

Last updated: 2000-03-24

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MYO5]

1) Geli MI and Riezman H  (1996) Role of type I myosins in receptor-mediated endocytosis in yeast. Science 272(5261):533-5
2) Anderson BL, et al.  (1998) The Src homology domain 3 (SH3) of a yeast type I myosin, Myo5p, binds to verprolin and is required for targeting to sites of actin polarization. J Cell Biol 141(6):1357-70
3) Tanaka K and Matsui Y  (2001) Functions of unconventional myosins in the yeast Saccharomyces cerevisiae. Cell Struct Funct 26(6):671-5
4) Brown SS  (1997) Myosins in yeast. Curr Opin Cell Biol 9(1):44-8
5) Goodson HV, et al.  (1996) Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton. J Cell Biol 133(6):1277-91
6) Pruyne D and Bretscher A  (2000) Polarization of cell growth in yeast. J Cell Sci 113 ( Pt 4):571-85
7) Botstein D, et al.  (1997) "The yeast cytoskeleton." Pp. 1-90 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
8) Goodson HV and Spudich JA  (1995) Identification and molecular characterization of a yeast myosin I. Cell Motil Cytoskeleton 30(1):73-84
9) Geli MI, et al.  (1998) Distinct functions of calmodulin are required for the uptake step of receptor-mediated endocytosis in yeast: the type I myosin Myo5p is one of the calmodulin targets. EMBO J 17(3):635-47
10) Mooseker MS and Cheney RE  (1995) Unconventional myosins. Annu Rev Cell Dev Biol 11():633-75