NAM7/YMR080C Summary Help

Standard Name NAM7 1
Systematic Name YMR080C
Alias IFS2 , MOF4 , UPF1 2 , SUP113 3
Feature Type ORF, Verified
Description ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress (4, 5, 6, 7, 8)
Name Description Nuclear Accommodation of Mitochondria 1
Chromosomal Location
ChrXIII:429627 to 426712 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 60 cM
Gene Ontology Annotations All NAM7 GO evidence and references
  View Computational GO annotations for NAM7
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
731 total interaction(s) for 652 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 27
  • Affinity Capture-RNA: 514
  • Affinity Capture-Western: 16
  • PCA: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 33
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 10
  • Positive Genetic: 42
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 3
  • Synthetic Rescue: 15

Resources
Expression Summary
histogram
Resources
Length (a.a.) 971
Molecular Weight (Da) 109,429
Isoelectric Point (pI) 6.45
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:429627 to 426712 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 60 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2916 429627..426712 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004685
References cited on this page View Complete Literature Guide for NAM7
1) Asher EB, et al.  (1989) Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria. Mol Gen Genet 215(3):517-28
2) Leeds P, et al.  (1991) The product of the yeast UPF1 gene is required for rapid turnover of mRNAs containing a premature translational termination codon. Genes Dev 5(12A):2303-14
3) Ono B, et al.  (2005) Suppression of termination mutations caused by defects of the NMD machinery in Saccharomyces cerevisiae. Genes Genet Syst 80(5):311-6
4) de la Cruz J, et al.  (1999) Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci 24(5):192-8
5) Sheth U and Parker R  (2006) Targeting of aberrant mRNAs to cytoplasmic processing bodies. Cell 125(6):1095-109
6) Takahashi S, et al.  (2008) Upf1 potentially serves as a RING-related E3 ubiquitin ligase via its association with Upf3 in yeast. RNA 14(9):1950-8
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Min EE, et al.  (2013) Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit. RNA 19(8):1105-15