VPS20/YMR077C Summary Help

Standard Name VPS20 1, 2
Systematic Name YMR077C
Alias CHM6 3 , VPT20 1 , VPL10 2
Feature Type ORF, Verified
Description Myristoylated subunit of the ESCRT-III complex; the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes (4, 5, 6 and see Summary Paragraph)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXIII:422149 to 421484 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VPS20 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
148 total interaction(s) for 104 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 13
  • FRET: 1
  • PCA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 73

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 8
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 4
  • Positive Genetic: 1
  • Synthetic Growth Defect: 18
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 12
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 221
Molecular Weight (Da) 25,638
Isoelectric Point (pI) 5.11
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:422149 to 421484 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..666 422149..421484 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004682
SUMMARY PARAGRAPH for VPS20

Most proteins targeted for degradation in the multivesicular body (MVB) pathway are monoubiquitinated, then passed through the series of highly-conserved multisubunit complexes known as ESCRT (Endosomal Sorting Complex Required for Transport). ESCRT I, II, and III are sequentially mobilized to endosomal membranes, where they direct protein sorting and MVB biogenesis, and play a crucial role in retrovirus budding (7).

The formation of ESCRT III - composed of subcomplexes Snf7p-Vps20p and Did4p-Vps24p - is transient and appears to be dependent on ESCRT II (8, 4). Snf7p-Vps20p effects the membrane association of ESCRT III, with Snf7p being required for localization of the Bro1p vacuolar protein sorting factor to endosomes (9, 10). Vps20p is myristoylated by Nmt1p, which is required for its localization to the endosomal membrane and for formation of ESCRT III (11, 4). Vps20p is also important for interaction between ESCRT III, ESCRT II, and ESCRT I, and may be involved in regulating the Vps4p AAA-type ATPase, which is necessary for the dissociation of ESCRT III (8, 5, 6, 7). Did4p-Vps24p is required for endosomal localization of Vps4p and the Doa4p ubiquitin hydrolase (4, 12).

Last updated: 2006-03-08 Contact SGD

References cited on this page View Complete Literature Guide for VPS20
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Howard TL, et al.  (2001) CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins. J Cell Sci 114(Pt 13):2395-404
4) Babst M, et al.  (2002) Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell 3(2):271-82
5) Yeo SC, et al.  (2003) Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae. J Cell Sci 116(Pt 19):3957-70
6) Bowers K, et al.  (2004) Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae. Traffic 5(3):194-210
7) Teo H, et al.  (2004) ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes. Dev Cell 7(4):559-69
8) Babst M, et al.  (2002) Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body. Dev Cell 3(2):283-9
9) Odorizzi G, et al.  (2003) Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae. J Cell Sci 116(Pt 10):1893-903
10) Boysen JH and Mitchell AP  (2006) Control of Bro1-domain protein Rim20 localization by external pH, ESCRT machinery, and the Saccharomyces cerevisiae Rim101 pathway. Mol Biol Cell 17(3):1344-53
11) Ashrafi K, et al.  (1998) A role for Saccharomyces cerevisiae fatty acid activation protein 4 in regulating protein N-myristoylation during entry into stationary phase. J Biol Chem 273(40):25864-74
12) Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80