ABF2/YMR072W Summary

ABF2/YMR072W Summary

Standard Name	ABF2 1 (see Nomenclature conflict Note)
Systematic Name	YMR072W
Feature Type	ORF, Verified
Description	Mitochondrial DNA-binding protein; involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation; ABF2 has a paralog, IXR1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	ARS-Binding Factor 1

Chromosomal Location	ChrXIII:411569 to 412120 \| ORF Map \| GBrowse

Gene Ontology Annotations All ABF2 GO evidence and references

	View Computational GO annotations for ABF2
Molecular Function
Manually curated	DNA binding (IDA) DNA binding, bending (IDA)
Biological Process
Manually curated	mitochondrial DNA packaging (IMP) mitochondrial genome maintenance (IMP) mitochondrion inheritance (IMP)
Cellular Component
Manually curated	mitochondrial chromosome (IDA) mitochondrial nucleoid (IDA)
High-throughput	mitochondrion (IDA)

Regulatory Role

Binding motifs

TCTAGA 5 [Search intergenic regions for TCTAGA]

Resources

ScerTF |
YeTFaSCo

Mutant phenotypes All ABF2 Phenotype evidence and references

Classical genetics
null	hyperosmotic stress resistance: decreased metal resistance: increased mitochondrial genome maintenance: abnormal mitochondrial morphology: abnormal resistance to DFD-VI-15: decreased
overexpression	budding index: abnormal cell cycle progression in G2 phase: abnormal
Large-scale survey
null	cell cycle progression in G1 phase: delayed glycogen accumulation: decreased glutathione excretion: increased competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to amitrole: decreased resistance to ethanol: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to pentachlorophenol: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased resistance to elesclomol-Cu: decreased resistance to cycloheximide: decreased respiratory growth: absent respiratory growth: decreased rate stress resistance: increased utilization of carbon source: decreased vegetative growth: decreased rate viable
overexpression	cell cycle progression: abnormal vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ABF2 Interaction evidence and references

	102 total interaction(s) for 74 unique genes/features.
Physical Interactions	Affinity Capture-MS: 56 Affinity Capture-RNA: 1 Affinity Capture-Western: 5 Co-fractionation: 1 PCA: 1
Genetic Interactions	Dosage Rescue: 5 Negative Genetic: 12 Phenotypic Enhancement: 2 Phenotypic Suppression: 2 Positive Genetic: 15 Synthetic Growth Defect: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ABF2 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIII:411569 to 412120 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..552	411569..412120	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004676

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
HIM1	Nomenclature conflict	HIM1 has been used to refer to both ABF2/YMR072W, which encodes a mitochondrial DNA-binding protein, and HIM1/YDR317W that plays a role in mismatch repair.

References cited on this page View Complete Literature Guide for ABF2

1)	Diffley JF and Stillman B (1991) A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria. Proc Natl Acad Sci U S A 88(17):7864-8
2)	MacAlpine DM, et al. (1998) The high mobility group protein Abf2p influences the level of yeast mitochondrial DNA recombination intermediates in vivo. Proc Natl Acad Sci U S A 95(12):6739-43
3)	Cho JH, et al. (2001) The modulation of the biological activities of mitochondrial histone Abf2p by yeast PKA and its possible role in the regulation of mitochondrial DNA content during glucose repression. Biochim Biophys Acta 1522(3):175-86
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Badis G, et al. (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87