KAR5/YMR065W Summary Help

Standard Name KAR5 1
Systematic Name YMR065W
Alias FIG3 2
Feature Type ORF, Verified
Description Protein required for nuclear membrane fusion during karyogamy; localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; similar to zebrafish Brambleberry protein; expression of the gene is regulated by pheromone (2, 3, 4, 5)
Name Description KARyogamy
Chromosomal Location
ChrXIII:399702 to 401216 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All KAR5 GO evidence and references
  View Computational GO annotations for KAR5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
overexpression
Resources
35 total interaction(s) for 28 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Co-localization: 1
  • PCA: 1
  • Two-hybrid: 9

Genetic Interactions
  • Negative Genetic: 18
  • Phenotypic Enhancement: 1
  • Positive Genetic: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 504
Molecular Weight (Da) 58,439
Isoelectric Point (pI) 4.63
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:399702 to 401216 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1515 399702..401216 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004669
References cited on this page View Complete Literature Guide for KAR5
1) Kurihara LJ, et al.  (1994) Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway. J Cell Biol 126(4):911-23
2) Erdman S, et al.  (1998) Pheromone-regulated genes required for yeast mating differentiation. J Cell Biol 140(3):461-83
3) Brizzio V, et al.  (1999) Genetic interactions between KAR7/SEC71, KAR8/JEM1, KAR5, and KAR2 during nuclear fusion in Saccharomyces cerevisiae. Mol Biol Cell 10(3):609-26
4) Beh CT, et al.  (1997) KAR5 encodes a novel pheromone-inducible protein required for homotypic nuclear fusion. J Cell Biol 139(5):1063-76
5) Abrams EW, et al.  (2012) Dynamic Assembly of Brambleberry Mediates Nuclear Envelope Fusion during Early Development. Cell 150(3):521-32