ARG7/YMR062C Summary

Standard Name	ARG7 1
Systematic Name	YMR062C
Alias	ECM40 2
Feature Type	ORF, Verified
Description	Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine (1 and see Summary Paragraph)
Name Description	ARGinine requiring

Chromosomal Location	ChrXIII:396379 to 395054 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All ARG7 GO evidence and references

	View Computational GO annotations for ARG7
Molecular Function
Manually curated	acetyl-CoA:L-glutamate N-acetyltransferase activity (IDA, IGI) glutamate N-acetyltransferase activity (IDA)
Biological Process
Manually curated	arginine biosynthetic process (TAS) ornithine biosynthetic process (IDA)
Cellular Component
Manually curated	mitochondrial matrix (IDA)
High-throughput	mitochondrion (IDA)

Pathways	arginine biosynthesis

Mutant phenotypes All ARG7 Phenotype evidence and references

Classical genetics
null	auxotrophy oxidative stress resistance: decreased toxin resistance: increased
Large-scale survey
null	competitive fitness: decreased resistance to quinine: decreased stress resistance: decreased toxin resistance: increased vegetative growth: decreased rate viable
unspecified	resistance to Calcofluor White: increased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ARG7 Interaction evidence and references

	54 total interaction(s) for 53 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1
Genetic Interactions	Negative Genetic: 23 Positive Genetic: 25 Synthetic Growth Defect: 2 Synthetic Lethality: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ARG7 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIII:396379 to 395054 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1326	396379..395054	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004666

SUMMARY PARAGRAPH for ARG7

ARG7 encodes the mitochondrial matrix enzyme glutamate N-acetyltransferase (also called ornithine acetyltransferase) (1, 3), which catalyzes the fifth step in the biosynthesis of ornithine, an intermediate in arginine biosynthesis (4). Deletion of ARG7 causes a leaky Arg^- phenotype, suggesting that yeast cells can generate ornithine from acetylornithine by an alternative route (1). ARG7p also has amino-acid N-acetyltransferase activity (1), and shows sequence similarity to glutamate N-acetyltransferases from several bacterial species (1). ARG7 was identified in a large-scale screen for mutants affecting cell surface assembly (2).

Last updated: 1999-10-21

References cited on this page View Complete Literature Guide for ARG7

1)	Crabeel M, et al. (1997) Characterization of the Saccharomyces cerevisiae ARG7 gene encoding ornithine acetyltransferase, an enzyme also endowed with acetylglutamate synthase activity. Eur J Biochem 250(2):232-41
2)	Lussier M, et al. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
3)	Liu Y, et al. (1995) Purification and characterization of ornithine acetyltransferase from Saccharomyces cerevisiae. Eur J Biochem 228(2):291-6
4)	Jauniaux JC, et al. (1978) Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes. J Bacteriol 133(3):1096-1107